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Reagents for detecting and targeting rp11-754b17.1 and their application in arthritis

A technology for osteoarthritis and reagents, which is applied in the field of reagents for detecting and targeting the biomarker RP11-754B17.1, which can solve the problems of no effective treatment, the influence of patients' families and society, and the inability to cure osteoarthritis.

Active Publication Date: 2020-09-15
XIAN HONGHUI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Osteoarthritis cannot be cured at present, and there is no effective treatment after the onset, which has a serious impact on patients, their families and society

Method used

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  • Reagents for detecting and targeting rp11-754b17.1 and their application in arthritis
  • Reagents for detecting and targeting rp11-754b17.1 and their application in arthritis
  • Reagents for detecting and targeting rp11-754b17.1 and their application in arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 QPCR sequencing to verify the expression of RP11-754B17.1 gene

[0055] 1. Sample collection

[0056] A total of 78 patients undergoing knee replacement surgery were selected, including 48 OA patients and 30 normal controls. Blood and tissue samples were collected from the patients. The age and gender of the OA group and the control group were not statistically significant.

[0057] OA group: Inclusion criteria: 1) According to the diagnostic criteria of OA, patients with osteoarthritis were diagnosed and underwent knee replacement surgery; 2) First replacement. Exclusion criteria: 1) Complicated with other inflammatory arthritis or autoimmune diseases, including rheumatoid arthritis, gout, systemic lupus erythematosus, etc.; 2) History of severe knee joint trauma; 3) Steroids in the past 3 months History of drug injection or non-steroid drug use; 4) Patients with severe liver and kidney disease and cardiovascular disease.

[0058] Control group: Inclusion criteria: ...

Embodiment 2

[0068] Example 2 Silencing of RP11-754B17.1 gene and its effect on cells

[0069] 1. Cell acquisition and culture

[0070] Wash the cartilage tissue of patients with OA 3 times with 1% bi-antibody PBS, and cut the tissue to 1 mm with sterile ophthalmic scissors 3 Put the obtained cartilage fragments into a l0cm petri dish, add l0ml fresh 0.25% trypsin, 37℃, 5% CO 2 Digest in an incubator for 30 minutes to remove fibroblasts that may adhere. Aspirate the trypsin digestion solution, add 10ml of DMEM culture solution containing 10% FBS, pipetting and mixing to terminate the digestion, and wash twice with 10ml of PBS containing double antibody. Add 10ml of 0.2% type II collagenase to the petri dish, place it at 37℃, 5% CO 2 Digest in the incubator for 4h. The free chondrocyte suspension containing collagenase solution was sucked out with a pipette, filtered through a 70 μm 200 mesh cell sieve, and the resulting filtrate was transferred into a centrifuge tube and centrifuged at 1000 r / ...

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PUM

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Abstract

The invention discloses a reagent for detecting and targeting at RP11-754B17.1 and application of the reagent in arthritis. The invention finds that RP11-754B17.1 is capable of effectively distinguishing patients suffering from bone arthritis from normal people, and meanwhile, cell proliferation can be promoted and cell apoptosis can be reduced by reducing the expression level of RP11-754B17.1, sothat a novel molecular means is provided for diagnosis and treatment on bone arthritis.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and relates to reagents for detecting and targeting biomarker RP11-754B17.1 and its application in arthritis. Background technique [0002] Osteoarthritis (OA) is a heterogeneous chronic disease with extremely complex pathogenic factors, which brings a huge economic burden to society. OA has a very high disability rate and severely reduces the quality of life of patients (Hunter DJ, Schofield D, Callander E. The individual and socioeconomic impact of osteoarthritis[J]. Nat Rev Rheumatol,2014,10(7):437-441 .), but there is still a lack of effective treatment. When OA occurs, a series of pathological changes occur in joint tissues: including cartilage degradation, synovial inflammation, osteophyte formation and subchondral bone sclerosis (Moon PM, Beier F. Novel Insights into Osteoarthritis Joint Pathology from Studies in Mice[J].Curr Rheumatol Rep, 2015, 17(8):50). Cartilage erosion is a sign of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883A61K45/00A61K31/7088A61P19/02A61P29/00
CPCA61K31/7088A61K45/00A61P19/02A61P29/00C12Q1/6883C12Q2600/136C12Q2600/158
Inventor 马涛王军宋伟程辉光张育民
Owner XIAN HONGHUI HOSPITAL