Extraction method of cacticin
An extraction method, cactus technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of serious environmental pollution, long time consumption, large sewage discharge, etc., achieve simple process, save solvent, even heating effect
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[0016] Example 1:
[0017] Prepare the cactus, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with a pH value of 4.5 at a ratio of 1:5, add 15% cellulase by mass after mixing, and hydrolyze at 55°C After 1 h, centrifuge at 4000 r / min for 10 min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, and add warm water (30 °C), 50% absolute ethanol, 0.05 mol / LPH value 3.7 at a material-to-liquid ratio of 1:5. The citric acid-disodium hydrogen phosphate buffer solution was extracted for 30min, and the material was placed in a microwave device for extraction under the same conditions for 12min. After the extraction, centrifuge at 4000r / min for 10min, take the supernatant, and combine the two supernatants , and dried at 60 °C to obtain cactoside, and the content of cactoside was 87.2% by HPLC detection.
Example Embodiment
[0018] Example 2:
[0019] Prepare soybean, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with pH value of 4.5 in a ratio of 1:5, add 15% cellulase by mass after homogenization, and hydrolyze at 55°C for 1 hour Then, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, add warm water (45°C), 55% absolute ethanol, 0.05mol / LPH value of 3.7 in a ratio of 1:5 Citric acid-disodium hydrogen phosphate buffer, extract for 35min, and under the same conditions, place the material in a microwave device to extract for 12min, after the extraction, centrifuge at 4000r / min for 10min, take the supernatant, combine the two supernatants, It was dried at 60°C to obtain cactoside, and the content of daidzein was 87.9% detected by HPLC.
Example Embodiment
[0020] Example 3:
[0021] Prepare soybean, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with pH value of 4.5 in a ratio of 1:5, add 15% cellulase by mass after homogenization, and hydrolyze at 55°C for 1 hour Then, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, add warm water (50°C), 60% anhydrous ethanol, 0.05mol / LPH value of 3.7 in the ratio of material to liquid 1:5 respectively. Citric acid-disodium hydrogen phosphate buffer, extract for 40min, and under the same conditions, place the material in a microwave device for extraction for 12min, after the extraction, centrifuge at 4000r / min for 10min, take the supernatant, combine the two supernatants, It was dried at 60°C to obtain daidzein, and the content of daidzein was 87.8% detected by HPLC.
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