Extraction method of cacticin

An extraction method, cactus technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of serious environmental pollution, long time consumption, large sewage discharge, etc., achieve simple process, save solvent, even heating effect

Inactive Publication Date: 2020-05-22
南京萌源康德生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, traditional methods are mostly used for the extraction of cactus glycosides, resulting in many organic by-produc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0016] Example 1:

[0017] Prepare the cactus, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with a pH value of 4.5 at a ratio of 1:5, add 15% cellulase by mass after mixing, and hydrolyze at 55°C After 1 h, centrifuge at 4000 r / min for 10 min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, and add warm water (30 °C), 50% absolute ethanol, 0.05 mol / LPH value 3.7 at a material-to-liquid ratio of 1:5. The citric acid-disodium hydrogen phosphate buffer solution was extracted for 30min, and the material was placed in a microwave device for extraction under the same conditions for 12min. After the extraction, centrifuge at 4000r / min for 10min, take the supernatant, and combine the two supernatants , and dried at 60 °C to obtain cactoside, and the content of cactoside was 87.2% by HPLC detection.

Example Embodiment

[0018] Example 2:

[0019] Prepare soybean, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with pH value of 4.5 in a ratio of 1:5, add 15% cellulase by mass after homogenization, and hydrolyze at 55°C for 1 hour Then, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, add warm water (45°C), 55% absolute ethanol, 0.05mol / LPH value of 3.7 in a ratio of 1:5 Citric acid-disodium hydrogen phosphate buffer, extract for 35min, and under the same conditions, place the material in a microwave device to extract for 12min, after the extraction, centrifuge at 4000r / min for 10min, take the supernatant, combine the two supernatants, It was dried at 60°C to obtain cactoside, and the content of daidzein was 87.9% detected by HPLC.

Example Embodiment

[0020] Example 3:

[0021] Prepare soybean, pulverize it, grind it into powder, add citric acid-disodium hydrogen phosphate buffer with pH value of 4.5 in a ratio of 1:5, add 15% cellulase by mass after homogenization, and hydrolyze at 55°C for 1 hour Then, centrifuge at 4000r / min for 10min, collect the supernatant, take an appropriate amount of the enzymatically broken wall precipitate, add warm water (50°C), 60% anhydrous ethanol, 0.05mol / LPH value of 3.7 in the ratio of material to liquid 1:5 respectively. Citric acid-disodium hydrogen phosphate buffer, extract for 40min, and under the same conditions, place the material in a microwave device for extraction for 12min, after the extraction, centrifuge at 4000r / min for 10min, take the supernatant, combine the two supernatants, It was dried at 60°C to obtain daidzein, and the content of daidzein was 87.8% detected by HPLC.

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Abstract

The invention relates to an extraction method for extracting cacticin from cactus, in particular to an extraction method adopting an enzymatic method-microwave wall breaking method. The extraction method includes the processing steps of: after the cactus is dried, the dried cactus is crushed in a crusher to obtain coarse cactus powder; and a certain amount of the coarse powder is weighed, a citricacid-disodium hydrogen phosphate buffer with a pH value of 4.5 is added at a material-solution ratio of 1:5, after mixing is carried out, 15% of cellulase is added by mass, after enzymolysis is carried out at 55 DEG C for 1h, centrifuging is carried out at 4000r/min for 10min, a supernatant is collected, appropriate amount of enzymatic-method wall-breaking sediment is taken, warm water, 50%-60% absolute ethyl alcohol, 0.05mol/L of a citric acid-disodium hydrogen phosphate buffer with a pH value of 3.7 are added at a material-solution ratio of 1:5, extracting is carried out for 30-40min, microwave-assisted extraction is carried out under the same conditions, after extraction is finished, centrifuging is carried out at 4000r/min for 10min, a supernatant is taken, and the two obtained supernatants are combined and dried at 60 DEG C to obtain the cacticin. Compared with a traditional extraction method, the extraction method has the advantages of simple operation, short time and reducing of decomposition of unstable substances.

Description

Technical field: [0001] The invention relates to a method for extracting cactus glycosides, which belongs to the technical field of extraction and separation of effective components of natural products. Background technique: [0002] Cactus glycoside is a class of active compounds with antibacterial properties. It also has antioxidant functions in medicine and has a wide range of application values. It can not only be used as feed additives for aquaculture and human food additives, but also in pharmaceuticals, cosmetics and Nutritional health products and other fields also have great application potential. [0003] Cactus is the plant with the highest content of cactus glycosides. According to the "Compendium of Materia Medica", cactus has the functions of promoting qi and blood circulation, clearing away heat and detoxification, relaxing tendons and blood circulation, and anti-oxidation and antibacterial effects. Among them, the substances that play anti-oxidation and antib...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08
CPCC07H1/08C07H17/07
Inventor 王俊
Owner 南京萌源康德生物技术有限公司
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