Supercharge Your Innovation With Domain-Expert AI Agents!

A kind of iridium complex its preparation method and application

A technology of iridium complexes and mixtures, which is applied in the direction of indium organic compounds, chemical instruments and methods, platinum group organic compounds, etc., can solve the problems of short luminous life, difficulty in shielding the background fluorescence of biological tissues, etc., and achieve simple preparation methods and good applications Prospects, effects of mild preparation conditions

Active Publication Date: 2022-06-07
LANZHOU UNIVERSITY +1
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although organic small molecule fluorescent probes have been reported for the detection of peroxynitrite (Coord.Chem.Rev.2018, 374(1), 36–54), most small molecule fluorescent probes are based on Fluorescent dyes are light-emitting units with short luminescence lifetimes, making it difficult to shield the interference of background fluorescence of biological tissues; in addition, active oxygen species such as hypochlorous acid and hydrogen peroxide have similar properties to peroxynitrite ions, and their differential detection is also facing challenges. certain challenges

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of iridium complex its preparation method and application
  • A kind of iridium complex its preparation method and application
  • A kind of iridium complex its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 (preparation of Ir-ONOO)

[0028] Step 1. Synthesis of intermediate dichloro-tetrakis(2-phenylpyridine)diiridium (III): a mixture of ethylene glycol ether and water (40 mL, volume ratio 3:1) was placed in a mixture containing IrCl 3 ·3H 2 O (0.353 g, 1 mmol) and 2-phenylpyridine (0.314 g, 2.2 mmol) in a flask; under argon, the mixture was stirred at 130 °C for 24 h; after cooling the mixture to room temperature, the yellow solid was filtered Precipitated, washed with water and ethanol; Yield: 89%, Yield: 0.954 g;

[0029] Step 2. Synthesis of intermediate 2-(4-nitrophenyl)-N-(1,10-o-phenanthrolin-5-yl)glyoxalamide: oxalyl chloride (252 μL, 3 mmol) and dry N, N-Dimethylformamide (100 μL) was sequentially added dropwise to a solution of p-nitrophenylglyoxylic acid (195 mg, 1 mmol) in anhydrous CH 2 Cl 2 The solution (10 mL) was stirred at room temperature for 2 h, and the solvent was removed by rotary evaporation to give a yellow solid. The above yellow sol...

Embodiment 2

[0036] Example 2 (using the iridium complex of Example 1 as a probe to detect peroxynitrite ions in aqueous solution)

[0037] 1. Iridium complex probe UV absorption experiment:

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an iridium complex and its preparation method and application. The iridium complex uses iridium trichloride as a raw material and is compounded with 2-phenylpyridine and 1,10-phenanthroline conjugated derivatives. Coordinated to obtain, labeled Ir‑ONOO. The invention provides a probe for detecting peroxynitrite ion in aqueous solution. The present invention also provides the application of the above-mentioned iridium complex in detecting peroxynitrite ion in aqueous solution. The iridium complex probe Ir-ONOO designed and synthesized by the present invention can identify peroxynitrite ions in the aqueous phase with single selectivity and high sensitivity. The iridium complex probe Ir-ONOO of the present invention has a long phosphorescence emission lifetime, uses time-resolved technology to shield the interference of background fluorescence, and is successfully applied to biological live cell imaging to effectively detect and track endogenous peroxynitrite in cells The content change of root ions has a good application prospect in the detection of clinical disease markers.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and relates to a preparation method and application of an iridium complex. Background technique [0002] peroxynitrite ion (ONOO - ) is one of the endogenous reactive oxygen species (ROS) with strong oxidizing and nucleophilic properties, composed of nitric oxide (NO) and superoxide anion radical (O 2 ·- ) is produced by a non-enzymatic diffusion reaction (J. Biol. Chem. 2013, 288(37), 26464-26472). Because peroxynitrite ion has strong oxidizing properties, it can react with a variety of biomolecules including proteins, lipids, nucleic acids and transition metal-containing enzymes. Damage to various molecular components including autoimmune diseases, inflammatory diseases, Alzheimer's disease, cancer and other diseases (Physiol. Rev. 2007, 87(1), 315-424; Nat . Rev. Drug Discov. 2007, 6(8), 662-680). Therefore, peroxynitrite ions can be used as biomarkers to detect va...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07F15/00C09K11/06G01N21/64
CPCC07F15/0033C09K11/06G01N21/6428G01N21/643C09K2211/185C09K2211/1044C09K2211/1029C09K2211/1007Y02E60/36
Inventor 唐晓亮李雪张航刘伟生李虎平
Owner LANZHOU UNIVERSITY
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com