Bacillus methylotrophicus and application thereof in degradation of micro-pollutants in environment
A technology of methyl bacillus and pollutants, applied in the field of methyl bacillus, can solve the problems of low biodegradation rate, less research on microbial degradation, long degradation time, etc., and achieve the effect of shortening the degradation time
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Embodiment 1
[0017] In the present invention, Bacillus methylotrophicus (Bacillus methylotrophicus) BP1.1 is screened and obtained from activated sludge in the biological aerobic section of a domestic sewage treatment plant in Nanjing.
[0018] The specific process is as follows:
[0019] (1) Take activated sludge from the biological aerobic section of a domestic sewage treatment plant in Nanjing;
[0020] (2) Get 250mL of the obtained mud-water mixture and put it in a conical flask, stir it at room temperature on a magnetic stirrer, add 5mg of 2,4-dihydroxybenzophenone to it every day; pour off the upper part before each dosing The supernatant (detect whether the supernatant contains the target pollutant-2,4-dihydroxybenzophenone), and then make up with tap water, so that the acclimatization is completed until the target pollutant is not detected in the solution;
[0021] (3) Take out the sludge obtained in step (2), apply the sludge to the inorganic salt culture medium containing 2,4-di...
Embodiment 2
[0029] The strains screened in Example 1 were identified.
[0030] In Example 1 of the present invention, the strain BP1.1 with the best degradation effect and the fastest growth rate was screened. Among them, BP1.1 was identified as Bacillus methylotrophicus.
[0031] Bacillus methylotrophicus (Bacillus methylotrophicus) BP1.1 grows well in LB medium under aerobic conditions at 30°C. The colonies are round, with a diameter of 0.2-1 mm. The color of the colonies is light pink, opaque, and the surface is dry. No creases. Gram staining was positive, and the cells were short rod-shaped under the microscope.
[0032] Through PCR amplification and Sanger method sequencing, the complete sequence of 16S rRNA of BP1.1 strain was obtained as follows (SEQ ID NO.1):
[0033]
[0034]
[0035] The BP1.1 sequencing results were submitted to GenBank for BLAST comparison. From the BLAST results, the strain sequences with a similarity of more than 98% to the BP1.1 base sequence were ...
Embodiment 3
[0037] Bacillus methylotrophicus (Bacillus methylotrophicus) BP1.1 of the present invention measures pollutant-2, the degradation rate of 4-dihydroxybenzophenone, and concrete steps are as follows:
[0038] (1) Centrifuge the bacterial liquid of BP1.1 at 6000rpm for 10min after expanded cultivation, and discard the supernatant to obtain the bacterial cells;
[0039] (2) Take the bacterium obtained in step (1) according to the inoculation amount of 1.8‰ (mass fraction), break up, add in the aqueous solution containing 2,4-dihydroxybenzophenone, in the aqueous solution, 2,4-dihydroxybenzophenone The mass concentration of benzophenone is 10 mg / L; and the treatment group without BP1.1 is used as a control;
[0040] (3) Put the control group and the treatment group in the step (2) into a shaker, and under aerobic conditions, shake and react at 150 rpm at 28°C;
[0041](4) The remaining concentrations of pollutants in the treatment group and the control group in the regular samplin...
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