Compounds with antitumor activity and their preparation methods and applications
A technology of anti-tumor activity and anti-tumor drugs, which is applied in the field of medicine to achieve the effect of reasonable process design, high purity and strong anti-tumor activity
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[0040] 1. A method of preparing a compound of the present invention:
[0041] (1) Granules of bran: Wheat bran and branger with weight ratio of 1: 4, will preheat the gun special pot, put the wheat bran into the pot, and put the gantry film and stir fry until the Atractylodes Yellow yellow, overflow aroma; the fried temperature is 200 ° C, the time is 26 min.
[0042] (2) Number of bran (1) bran (1) bran, crushing after 40 mesh sieve, take at the atractylodes of 2.0kg, extract 2 times with 10L, 8L methanol, 2 hours each time, combine extract, minus The solvent was pressed, and the extract was 208.7 g after drying.
[0043] (3) After adding 2 L of the extract from the extract obtained by step (2), 2 L of n-hexane is added 2 times, combined with the extract, and the solvent is removed, and the n-hexane extract component is 40.37 g.
[0044] (4) Silica gel column chromatography in step (3): Silica 820g, Catalog # 40930-25, L26INCH * D3.5 inch, mixed solvent using n-hexane-chloroform ...
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