Separation and purification method of lactobacillus paracasei ZFM54 bacteriocin
A technology for separation and purification of Lactobacillus, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., and can solve problems such as inability to accurately detect, difficulty in separation and purification, and low concentration of bacteriocins
Active Publication Date: 2020-06-16
顾容铖
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Problems solved by technology
Bacteriocins from lactic acid bacteria are metabolites of extracellular secretion. However, due to the low concentration of bacteriocins in the fermentation supernatant, it is difficult to directly separate and purify them, and may not be accurately detected during the detection stage. Therefore, the fermentation supernatant is generally first Crude extraction to concentrate, followed by subsequent separation and purification
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[0052] The following description serves to disclose the present invention to enable those skilled in the art to carry out the present invention. The preferred embodiments described below are only examples, and those skilled in the art can devise other obvious variations. The basic principles of the present invention defined in the following description can be applied to other embodiments, variations, improvements, equivalents and other technical solutions without departing from the spirit and scope of the present invention.
[0053] Those skilled in the art should understand that in the disclosure of the present invention, the terms "vertical", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientation or positional relationship indicated by "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, which are only for the convenience of describing the present invention...
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Abstract
The invention discloses a separation and purification method of lactobacillus paracasei ZMF54 bacteriocin. The separation and purification method comprises the following steps: (A) obtaining lactobacillus paracasei ZFM54; (B) determining bacteriocin titer; (C) determining the optimal fermentation conditions of the lactobacillus paracasei ZFM54 for producing antibacterial substances; (D) preparinga fermented supernatant; (E) carrying out macroporous resin adsorption chromatography; (F) carrying out ion exchange chromatography; (G) carrying out gel filtration chromatography; and (H) carrying out preparative HPLC purification, wherein the lactobacillus paracasei ZMF54 bacteriocin is obtained through the multi-step separation and purification method.
Description
technical field [0001] The invention relates to the field of strain culture, and further relates to a separation and purification method of Lactobacillus paracasei ZMF54 bacteriocin. Background technique [0002] The current commercialized lactobacillus Nisin is unstable to heat and has a narrow antibacterial spectrum. It can effectively inhibit the growth of Gram-positive bacteria but has no inhibitory effect on the growth of most Gram-negative bacteria and molds. It is widely used in the food industry. Applications in are restricted. Therefore, the development of broad-spectrum and high-efficiency bacteriocins from lactic acid bacteria as natural preservatives has become a research hotspot and has attracted much attention. [0003] Lactobacillus paracasei is often used as a starter and auxiliary starter for dairy products such as milk, yogurt, cream and cheese. Bacteriocins are generally defined as a class of small molecular proteins or polypeptides synthesized by riboso...
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IPC IPC(8): C07K14/335C07K1/18C07K1/16
CPCC07K14/335
Inventor 顾容铖顾青
Owner 顾容铖