Preparation method of fermentation liquor containing rhamnolipid
A technology of rhamnolipid and fermentation liquid, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions, can solve the problems of low product yield, small fermentation scale and high production cost, and achieve high product yield , The effect of low production cost and easy process
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[0028] A preparation method for a fermented liquid containing rhamnolipids, comprising the following steps:
[0029] 1) Inoculating the rhamnolipid-producing bacterial strain into the seed culture medium at a ratio of 1-3% for expanded culture to obtain the seed bacteria fermentation liquid.
[0030] 2) Inoculate the fermented liquid of the seed bacteria into the sterilized fermenter culture medium with an inoculation amount of 4-5%. The fermenter culture medium contains at least one of fish oil, camphor tree oil and palm oil.
[0031] 3) Controlling the pH value in stages during the fermentation process, adding carbon sources at the same time, and obtaining a fermented liquid containing rhamnolipids through aerated fermentation.
[0032] As a preference, a strain of Pseudomonas aeruginosa with high rhamnolipid production, which is named zs1.1, has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on December 09,...
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[0038] Preparation of seed medium: Mineral salt medium (MSM)+2% yeast powder (mass volume ratio), take Pseudomonas aeruginosa zs1.1 in the glycerol tube and insert the seed medium at a ratio of 2%, at 30°C , and cultured for 7 hours under the condition of a shaking table with a rotating speed of 180r / min.
[0039] Preparation of fermentation medium: fish oil 15g / L, camphor oil 15g / L, palm oil 10g / L, NaNO 3 5.43g / L, NH 4 NO 3 2.56g / L, Na 2 PO 4 10g / L, KH 2 PO 4 7.7g / L, MgSO 4 ·7H 2 O0.3g / L, CaCl 2 10.01g / L, trace element solution 3mL / L (FeSO 4 ·7H 2 O18g / L; ZnSO 4 ·7H 2 O3.0g / L; MnSO 4 2H 2 (03.0g / L), yeast powder 0.5g / L.
[0040] Fill 30L of fermentation medium into a 50L tank, adjust the initial pH value of the medium to 7, and adopt vertical in-situ sterilization. The initial conditions are: rotating speed 300rpm, dissolved oxygen 45%, tank pressure about 0.04mPa.
[0041] The expanded seed bacteria fermentation liquid is inoculated into the sterilized f...
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