Method and kit for detecting paraquat and/or diquat in blood sample

A blood sample, paraquat technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome processing and unsuitable for wide application, and achieve simple operation methods, good practical application value, preparation and testing price cheap effect

Pending Publication Date: 2020-06-16
济南爱新卓尔医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the above methods are reported for the monitoring of herbicide content in the environment, and there are few methods for the detect

Method used

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  • Method and kit for detecting paraquat and/or diquat in blood sample
  • Method and kit for detecting paraquat and/or diquat in blood sample
  • Method and kit for detecting paraquat and/or diquat in blood sample

Examples

Experimental program
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Example Embodiment

[0060] Example

[0061] 1. Equipment and reagents

[0062] 1.1 Main equipment

[0063]

[0064] 1.2 Reagents

[0065] Methanol (Merck, HPLC)

[0066] Acetonitrile (Merck, HPLC)

[0067] Ammonium formate (Sigma, LC-MS / MS)

[0068] Formic acid (Sigma, LC-MS / MS)

[0069] Water: Ultrapure water (Watsons).

[0070] 2. Abbreviations

[0071] abbreviation illustrate abbreviation illustrate BCK Paraquat BCK-d8 Paraquat-d8 internal standard DCK diquat

[0072] 3. LC / MS / MS analysis method

[0073] 3.1 Liquid chromatography conditions

[0074] Chromatographic column: Thermo Hypersil GOLD aQ column (100mm*2.1mm, 1.9μm).

[0075] Mobile phase A: containing 0.2% formic acid-5mmol / L ammonium formate aqueous solution, mixed by vortex for 1min, then ultrasonically mixed for 10min, filtered with a 0.22 μm membrane (water membrane), and degassed by ultrasonic for 10mins; mobile phase B: containing 0.2% formic acid- Methanol solution, vortex...

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Abstract

The invention provides a method and a kit for detecting paraquat and/or diquat in a blood sample, and belongs to the technical field of pesticide detection substance analysis. A direct protein precipitation method is established for extraction; the invention discloses a liquid chromatography-tandem mass spectrometry method. Through optimizing a sample pretreatment method, liquid chromatography conditions and mass spectrometry conditions, the method for detecting paraquat and/or diquat in the blood sample is established successfully, and the method is rapid, accurate in result, simple and convenient in operation method, good in sensitivity, strong in specificity, relatively low in preparation and detection cost, and suitable for screening and quantitative detection of poisons in clinical poisoned patients, so that the method has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of analysis of pesticide detection substances, and specifically provides a method and a kit for detecting paraquat and / or diquat in blood samples. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Paraquat (paraquat), chemical name 1,1-dimethyl-4,4-bipyridine dichloride, diquat (Diquat), also known as diquat, double fast, chemical name 1,1' -Ethylene-2,2'-bipyridyl dibromide, the two have similar chemical properties, both belong to the bipyridyl quaternary ammonium salt, and are a cheap, fast-acting, non-selective contact-killing herbicide. Among them, paraquat is highly toxic, and currently ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/062
Inventor 张义张欣胡志恒张振红孔金隆徐晓阳
Owner 济南爱新卓尔医学检验有限公司
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