Inhibitor for inhibiting production of vomitoxin by fusarium graminearum
A technology of Fusarium graminearum and vomitoxin, which is applied in the field of inhibitors for inhibiting the production of vomitoxin by Fusarium graminearum, can solve problems such as food safety problems, difficulties in large-scale promotion and application, etc., and achieve improved extraction efficiency and improved extraction time. Effect of shortening and high stability
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Embodiment 1
[0027] Embodiment 1 A kind of preparation of nanoemulsion inhibitor
[0028] (1) Beat 200g of fresh bergamot, add 1000ml of pure water, and extract the volatile oil by microwave-assisted distillation; the microwave power is 600W, and the extraction time is 1.5h, and the volatile oil is collected;
[0029] (2) Lactoferrin is dissolved in neutral phosphate buffer to obtain a solution with a protein concentration of 0.5%. Get bergamot volatile oil 0.1g and 0.6g camellia oil and mix evenly to form a mixed oil. Mix the mixed oil with 4.3g The protein solutions were mixed, ultrasonically treated for 5 minutes, and then homogenized for 20 minutes with a high-pressure homogenizer under the condition of 50Mpa to obtain a lactoferrin-based bergamot volatile oil nanoemulsion.
[0030] The obtained nanoemulsion inhibitor is placed at room temperature for more than 30 days, and its properties do not change, it can be miscible with water in any proportion, and its stability is very good. I...
Embodiment 2
[0031] Embodiment 2 Preparation of a nanoemulsion inhibitor
[0032] (1) Grind 100g of dried bergamot, add 3L of pure water, and extract the volatile oil by microwave distillation; the microwave power is 900W, the extraction time is 2h, and the volatile oil is collected;
[0033] (2) Lactoferrin is dissolved in neutral phosphate buffer to obtain a solution with a protein concentration of 0.3%. 1.0g bergamot volatile oil is mixed with 5.3g camellia oil to form a mixed oil, and the mixed oil is mixed with 33g protein The solutions were mixed, ultrasonically treated for 10 minutes, and then homogenized for 15 minutes with a high-pressure homogenizer under the condition of 75Mpa to obtain the bergamot volatile oil nanoemulsion.
[0034] The obtained nanoemulsion inhibitor is placed at room temperature for more than 30 days, and its properties do not change, it can be miscible with water in any proportion, and its stability is very good. In specific applications, it can be diluted...
Embodiment 3
[0035] Embodiment 3 Preparation of a nanoemulsion inhibitor
[0036] (1) Beat 2kg of fresh bergamot, add 15L of pure water, and extract the volatile oil by microwave distillation; the microwave power is 1500W, the extraction time is 1.5h, and the volatile oil is collected;
[0037] (2) Lactoferrin is dissolved in neutral phosphate buffer to obtain a solution with a protein concentration of 0.2%. 0.5g bergamot volatile oil is mixed with 2g camellia oil to form a mixed oil, and the mixed oil is mixed with 12g protein solution Mix and ultrasonically treat for 20 minutes, and then use a high-pressure homogenizer to homogenize for 6 minutes under the condition of 80Mpa to obtain bergamot volatile oil nanoemulsion.
[0038] The obtained nanoemulsion inhibitor is placed at room temperature for more than 30 days, and its properties do not change, it can be miscible with water in any proportion, and its stability is very good. In specific applications, it can be diluted multiple times...
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