Application of water-soluble fullerene structure to preparation of medicine for treating Parkinson's disease
A fullerene structure, Parkinson's disease technology, applied in the field of medicine, can solve the problem of no good treatment for PD
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Embodiment 1
[0062] Embodiment 1, water-soluble modified hollow fullerene C 70 Preparation of -OH
[0063] (a) Add 7mL of 30% aqueous hydrogen peroxide and 3mL of 40% sodium hydroxide solution into a 100mL round bottom flask by mass, add 200mg of fullerene C 70 Solid, then add a magnetic stirrer, use a magnetic stirrer to stir at a temperature of 50°C and a speed of 1000r / min for 24h, use a solvent filter (volume: 1L, filter membrane pore size: 200nm, Jinteng Company) to filter to obtain a brownish yellow color solution.
[0064] (b) Add the solution obtained in step (a) into a 50ml centrifuge tube, and then add excess ethanol with a concentration of 95%. After centrifuging at a speed of 10000r / min for 4min, the colorless solution in the upper layer was removed, and the collected precipitate was dissolved in ultrapure water to obtain a yellow clear solution.
[0065] (c) Put the solution obtained in step (b) into a dialysis bag with a molecular weight cut-off of 3500, put it into ultrap...
Embodiment 2
[0069] Embodiment 2, fullerene C 70 -OH free radical scavenging ability test
[0070] The present invention detects water-soluble modified hollow fullerene C by electron spin resonance spectroscopy (ESR) 70 The ability of -OH to scavenge free radicals.
[0071] Detection method: UV-induced generation of hydroxyl radicals, 50 μL of 39% hydrogen peroxide, 50 μL of PBS buffer solution (PH=7.4) and a small amount (0.133 mM) of lutidine N-oxide (DMPO, free base capture agent) solution mixed, the control group was directly irradiated with ultraviolet light, while the experimental group was immediately added 20 μL of C 70 -OH, irradiated with 280nm ultraviolet light for 4min, and detected the free radical signal. like image 3 Shown, the dotted line is not adding the obtained C of Example 1 70 The blank control of -OH, the non-dotted line is the addition of C 70 -OH samples, compared with the blank control, adding C 70 The signal of the -OH sample was significantly reduced, in...
Embodiment 3
[0072] Embodiment 3, animal experiments
[0073] (1) Establishment and treatment of Parkinson's disease model
[0074] The present invention adopts the mature MPTP (1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridinehydrochloride)-induced Parkinson's disease model in mice. Studies have found that C57BL / 6 mice are the most sensitive to MPTP, so the present invention uses C57BL / 6 mice for experiments.
[0075] In the present invention, 30 C57BL / 6 mice, 10 weeks old and weighing 18-22 g, were randomly divided into control group A, model group B and treatment group C, with 10 mice in each group.
[0076] 20 mice in two groups B and C were subcutaneously injected with MPTP to establish a model, and the injection amount of MPTP was 25 mg / kg, injected once a day for 5 consecutive days. The mice in groups B and C showed resting tremor and reduced movement. Vertical tail, vertical hair, etc., prove that the model is built successfully. Treatment started on day 6 and continued until day 14....
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