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Method for extracting vitamin B from yeast residues

A technology of yeast residue and vitamins, applied in food science, application, food ingredients, etc., can solve environmental pollution and other problems, and achieve good extraction effect, high extraction efficiency, and high purity

Inactive Publication Date: 2020-06-26
浙江唯娜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, many beer companies in my country put waste yeast directly into the environment, causing serious environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Crush the yeast residue in a pulverizer to below 200 mesh to remove visible impurities on the surface; add 100kg of crushed yeast residue to 500kg of water, stir evenly, and place it in a water bath at a temperature of 65°C for 15 minutes, stirring continuously during this period; HCl solution with a ratio of 0.1mol / L, stir well, adjust the pH to 3-4 for acid hydrolysis, ultrasonic extraction for 30min; repeat the extraction of centrifuged sediment twice, and combine with the extract of step (3) to obtain the B vitamin extract ;Use the above-mentioned obtained treatment solution to perform ultrafiltration using a hollow fiber filter membrane with a filter hole diameter of 0.01 μm to obtain a filtrate; carry out vacuum concentration of the above-mentioned obtained filtrate, and then cool the concentrated solution to reduce its solubility, and precipitate vitamin B crystals; finally The B vitamins are obtained by freeze-drying.

Embodiment 2

[0025] Crush the yeast residue in a pulverizer to below 200 mesh to remove visible impurities on the surface; add 200kg of crushed yeast residue to 400 parts of water, stir evenly, and place it in a water bath at 80°C for 15 minutes, stirring continuously; add A certain proportion of 0.1mol / L HCl solution, stir well, adjust the pH to 4 for acid hydrolysis, ultrasonic extraction for 30min; repeat the extraction of centrifuged sediment twice, and merge with the extract of step (3) to obtain the B vitamin extract; Ultrafiltration is performed on the above obtained treatment solution using a hollow fiber filter membrane with a filter pore diameter of 0.01 μm to obtain a filtrate; the above obtained filtrate is vacuum concentrated, and then the concentrated solution is cooled to reduce its solubility, and vitamin B crystals are precipitated; finally, B vitamins can be obtained by freeze-drying.

Embodiment 3

[0027] Crush the yeast residue in a pulverizer to below 200 mesh to remove visible impurities on the surface; add 150kg of crushed yeast residue to 500kg of water, stir evenly, and place in a water bath at a temperature of 55°C for 15 minutes, stirring continuously; add 0.1 mol / L HCl solution, stir well, adjust the pH to 5 for acid hydrolysis, ultrasonic extraction for 30min; repeat the extraction of the centrifuged sediment twice, and merge with the extract of step (3) to obtain the B vitamin extract; The treatment solution is ultrafiltered with a hollow fiber filter membrane with a filter hole diameter of 0.01 μm to obtain a filtrate; the above-mentioned filtrate is concentrated in a vacuum, and then the concentrated solution is cooled to reduce its solubility, and vitamin B crystals are precipitated; finally, it is freeze-dried to obtain Get B vitamins.

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Abstract

The invention discloses a method for extracting vitamin B from yeast residues. The method comprises the following steps: crushing yeast residues to 200 meshes or below, removing impurities, adding a certain proportion of water, performing uniform stirring, adding a certain proportion of HCl for acid hydrolysis after high-temperature water bath, and performing ultrasonic extraction to obtain a B group vitamin extract liquid; and performing ultrafiltration, vacuum concentration and freeze drying to obtain vitamins B of the yeast residues. According to the method for extracting vitamin B from theyeast residues, the structure of yeast cells is crushed by ultrasonic extraction, and most of vitamin B can be released from the yeast cells. The method is more efficient than direct water extraction, and has a better extraction effect. The method provided by the invention has a simple technical scheme, is low in cost and is easy for industrialized popularization.

Description

technical field [0001] The invention relates to the technical field of biomedicine preparation, in particular to a method for extracting vitamin B from yeast residue. Background technique [0002] Vitamin B includes vitamin B1, vitamin B2, vitamin B6, vitamin B12, niacin, pantothenic acid, folic acid and so on. B vitamins are indispensable substances to promote metabolism in the body and convert sugar, fat, protein, etc. into heat. If there is a lack of vitamin B, the cell function will immediately decrease, causing metabolic disorders. At this time, the human body will appear sluggish and loss of appetite. In addition, liver damage caused by drinking too much alcohol is parallel to vitamin B deficiency in many cases. Supplementing with vitamin B complex can soothe the mood. Therefore, it must be supplemented from some foods rich in B vitamins, such as yeast, rice bran, bran, yeast, animal liver, vegetables and other foods. [0003] During the brewing process of beer, a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/15
CPCA23L33/15A23V2002/00A23V2250/704
Inventor 王雷缪铭王玉涛
Owner 浙江唯娜科技有限公司