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A kind of method utilizing biological fermentation to extract dendrobium officinale polysaccharide

A technology of dendrobium candidum polysaccharides and dendrobium candidum polysaccharides, applied in the field of extracting polysaccharides from dendrobium candidum, can solve the problems of unsatisfactory yield of polysaccharides from dendrobium candidum, and achieve the effects of easy separation, improved fermentation efficiency, improved yield and decolorization rate

Active Publication Date: 2020-11-13
泉后(广州)生物科技研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The problem with this patent is that the yield of extracting Dendrobium officinale polysaccharide is not ideal

Method used

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  • A kind of method utilizing biological fermentation to extract dendrobium officinale polysaccharide
  • A kind of method utilizing biological fermentation to extract dendrobium officinale polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The method utilizing biological fermentation to extract the polysaccharide of dendrobium officinale comprises the following steps:

[0028] S1. Cut the stems of Dendrobium candidum into 2cm long stem sections, place them in an oven and dry them for 18 hours at 55°C, and grind them to 50 mesh with a high-speed pulverizer to obtain Dendrobium candidum powder;

[0029] S2. Add the Dendrobium officinale powder to 55 times the weight of water, process it with a colloid mill for 15 minutes and add a high-pressure homogenizer for homogenization for 20 minutes, take it out and pass through an 80-mesh sieve, and obtain the Dendrobium officinale fine powder after sterilization; homogeneous treatment During the process, the pressure is 25Mpa, the time is 20 minutes, the spray drying inlet air temperature is 180°C, the outlet air temperature is 90°C, the feed temperature is 60°C, and the feed rate is 10mL / min;

[0030] S3. Inoculate the culture medium with a volume of 2% of the sac...

Embodiment 2

[0036] The method utilizing biological fermentation to extract the polysaccharide of dendrobium officinale comprises the following steps:

[0037] S1. Cut the stems of Dendrobium candidum into 3cm long stem sections, place them in an oven and dry them for 24 hours at 50°C, and grind them to 50 mesh with a high-speed pulverizer to obtain Dendrobium candidum powder;

[0038] S2. Add the Dendrobium officinale powder to 55 times the weight of water, process it with a colloid mill for 15 minutes and add a high-pressure homogenizer for homogenization for 20 minutes, take it out and pass through an 80-mesh sieve, and obtain the Dendrobium officinale fine powder after sterilization; homogeneous treatment During the process, the pressure is 25Mpa, the time is 20 minutes, the spray drying inlet air temperature is 180°C, the outlet air temperature is 90°C, the feed temperature is 60°C, and the feed rate is 10mL / min;

[0039] S3. Inoculate the culture medium with a volume of 1% of the sac...

Embodiment 3

[0044]The method utilizing biological fermentation to extract the polysaccharide of dendrobium officinale comprises the following steps:

[0045] S1. Cut the stems of Dendrobium candidum into 2cm long stem sections, place them in an oven and dry them for 15 hours at 60°C, and grind them to 50 mesh with a high-speed pulverizer to obtain Dendrobium candidum powder;

[0046] S2. Add the Dendrobium officinale powder to 55 times the weight of water, process it with a colloid mill for 15 minutes and add a high-pressure homogenizer for homogenization for 20 minutes, take it out and pass through an 80-mesh sieve, and obtain the Dendrobium officinale fine powder after sterilization; homogeneous treatment During the process, the pressure is 25Mpa, the time is 20 minutes, the spray drying inlet air temperature is 180°C, the outlet air temperature is 90°C, the feed temperature is 60°C, and the feed rate is 10mL / min;

[0047] S3. Inoculate the culture medium on the yeast with a volume of 1...

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Abstract

The present invention provides a method for extracting dendrobium officinale polysaccharides by biological fermentation. The method comprises the following steps: S1, cutting stems of dendrobium officinale into stem segments, placing the stem segments into an oven for drying and crushing the dried stem segments by using a high-speed crusher to obtain dendrobium officinale powder; S2, adding the dendrobium officinale powder to water, treating the material with a colloid mill, adding the treated material to a high-pressure homogenizer for a homogenization treatment, taking out the homogenized material for sieving, and obtaining dendrobium officinale fine powder after sterilization; S3, inoculating a culture medium with yeasts, obtainin culture liquid after shaking flask culture, feeding theobtained dendrobium officinale fine powder in the step S2, and conducting continuous culture to obtain fermentation liquid; S4, conducting centrifuging separation of the fermentation liquid obtained in the step S3 to obtain supernatant; and S5, diluting the supernatant obtained in the step S4, loading samples into resin packing in an adsorption column, conducting elution and collecting an eluent,adding the eluent to absolute ethanol, conducting stirring until a precipitate is produced, and conducting centrifuging separation to obtain the precipitate after still-putting, and conducting dryingto obtain dendrobium officinale polysaccharides. The extracted dendrobium officinale polysaccharides are relatively high in yield, protein removal rate and decolorization rate.

Description

technical field [0001] The invention relates to a method for extracting dendrobium officinale polysaccharide by biological fermentation. Background technique [0002] Dendrobium officinale is a kind of Dendrobium orchidaceae plant, and it is a traditional Chinese medicinal material in my country. Dendrobium officinale has been known as "the gold in medicine" since ancient times. The Taoist medical classic "Tao Zang" listed Dendrobium officinale as the first of the "Nine Immortal Herbs", and the folks called it "Life-saving Immortal Herb". Dendrobium candidum has strict requirements on the growth environment, difficult cultivation, and people's over-harvesting of Dendrobium candidum has led to the endangered resources of Dendrobium candidum. Dendrobium candidum has been listed as a national second-class protected endangered plant. Studies in recent years have shown that the main active ingredients in Dendrobium officinale are Dendrobium polysaccharides, Dendrobine, amino aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00
CPCC08B37/0003C12P19/04
Inventor 黄小龙张娇韩志东
Owner 泉后(广州)生物科技研究院有限公司