Neutrophil gelatin related lipocalin detection kit and use method thereof
A technology of lipocalin and neutrophils, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of unfavorable high-throughput automatic detection, poor specificity, low sensitivity, etc., and achieve easy promotion and variation The effect of small, high signal sensitivity
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Embodiment 1
[0056] The components of the calibrator and the quality control are the same, and both are prepared by dissolving NGAL antigen in a buffer containing protein and diluting it into a buffer containing Tris; wherein, the buffer containing protein includes the following by volume percentage Components: BSA6%, preservative tetracycline 0.01% and neomycin sulfate 0.1%; Tris-containing buffer includes tetracycline 0.01% and neomycin sulfate 0.1% by volume percentage; and protein-containing buffer and containing All Tris buffers are filtered through a 0.22μm filter membrane;
[0057] The anti-reagent is prepared by an operation comprising the following steps:
[0058] A: Preparation of fluorescein isothiocyanate and NGAL antibody conjugates:
[0059] Sa1: Prepare fluorescein isothiocyanate to a concentration of 1.0 mg / mL with anti-reagent buffer solution, add NGAL antibody under light-shielding conditions, and stir at room temperature for 1 h; wherein, the anti-reagent buffer solutio...
Embodiment 2
[0072] The components of the calibrator and the quality control are the same, and both are prepared by dissolving NGAL antigen in a buffer containing protein and diluting it into a buffer containing Tris; wherein, the buffer containing protein includes the following by volume percentage Components: BSA6%, preservative tetracycline 0.03% and neomycin sulfate 0.3%; Tris-containing buffer includes tetracycline 0.03% and neomycin sulfate 0.3% by volume percentage; and protein-containing buffer and containing All Tris buffers are filtered through a 0.22μm filter membrane;
[0073] The anti-reagent is prepared by an operation comprising the following steps:
[0074] A: Preparation of fluorescein isothiocyanate and NGAL antibody conjugates:
[0075] Sa1: Prepare fluorescein isothiocyanate to a concentration of 3.0 mg / mL with anti-reagent buffer, add NGAL antibody under light-shielding conditions, and stir at room temperature for 1.5 h; wherein, the anti-reagent buffer has a pH value...
Embodiment 3
[0088] The components of the calibrator and the quality control are the same, and both are prepared by dissolving NGAL antigen in a buffer containing protein and diluting it into a buffer containing Tris; wherein, the buffer containing protein includes the following by volume percentage Components: BSA6%, preservative tetracycline 0.05% and neomycin sulfate 0.5%; Tris-containing buffer includes tetracycline 0.05% and neomycin sulfate 0.5% by volume percentage; and protein-containing buffer and containing All Tris buffers are filtered through a 0.22μm filter membrane;
[0089] The anti-reagent is prepared by an operation comprising the following steps:
[0090] A: Preparation of fluorescein isothiocyanate and NGAL antibody conjugates:
[0091] Sa1: Prepare fluorescein isothiocyanate to a concentration of 5.0 mg / mL with anti-reagent buffer, add NGAL antibody under light-shielding conditions, and stir at room temperature for 2 hours; wherein, the anti-reagent buffer is a PBS sys...
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Abstract
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