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Preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome

A technology of glucose-based and lutein lipids, which is applied in liposome delivery, medical preparations of non-active ingredients, pharmaceutical formulas, etc., can solve problems such as shortened half-life of blood, and achieve improved slow-release performance, improved absorption, and storage Highly stable effect

Active Publication Date: 2020-06-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, long-term use of PEG-modified liposomes will cause the human body to produce anti-PEG IgG, and the immunogenicity of PEG will cause an obvious humoral immune response, resulting in a significant shortening of the blood half-life of PEG-modified liposomes.

Method used

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  • Preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome

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Embodiment 1

[0027] Lutein, cholesterol and dipalmitoylphosphatidylcholine were dissolved in chloroform-methanol solution with a volume ratio of 2:1 to obtain a solution containing 0.1% lutein lipid. The organic solvent was removed by rotary evaporation, so that the compound formed a uniform film at the bottom of the round-bottomed flask, and the vacuum degree was increased to 0.1 MPa for 30 min. Add a phosphate solution to the composite film for hydration, the hydration temperature is 60°C, and the time is 120min; under the condition of stirring in an ice-water bath, ultrasonic, the ultrasonic power is 800W, the pulse is 5s / 5s, after the treatment time is 20min, continue to stir at 30°C for 3h , the stirring speed is 1000r / min; the dispersion liquid is centrifuged at 10000r / min for 10min to remove unembedded lutein. α-Glucosyl hesperidin solution (0.5%) was added dropwise to the dispersion, and stirred overnight at room temperature. The resulting dispersion was squeezed 10 times through ...

Embodiment 2

[0031] Lutein, cholesterol and dipalmitoylphosphatidylcholine were dissolved in a chloroform-methanol solution with a volume ratio of 2:1 to obtain a 2% lutein lipid solution. The organic solvent was removed by rotary evaporation, so that the compound formed a uniform film at the bottom of the round-bottomed flask, and the vacuum degree was increased to 0.1 MPa for 30 min. Add a phosphate solution to the composite film for hydration, the hydration temperature is 55°C, and the time is 100min; ultrasonication is performed under the condition of stirring in an ice-water bath, the ultrasonic power is 800W, the pulse is 5s / 5s, and after the treatment time is 30min, the stirring is continued at 40°C for 3h , the stirring speed is 1000r / min; the dispersion liquid is centrifuged at 10000r / min for 10min to remove unembedded lutein. α-Glucosyl hesperidin solution (10%) was added dropwise to the dispersion, and stirred overnight at room temperature. The resulting dispersion was squeezed...

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Abstract

The invention discloses a preparation method of alpha-glucosyl hesperidin modified xanthophyll liposome. The xanthophyll liposome provided by the invention is obtained by modification with alpha-glucosyl hesperidin, and the specific method is as follows: (1) dissolving xanthophyll and a lipid material to obtain a xanthophyll lipid solution; (2) hydrating a lipid membrane, and then performing ultrasonic treatment to obtain a dispersion solution; (3) dropwise adding an alpha-glucosyl hesperidin solution into the dispersion solution, and performing stirring at room temperature overnight; and (4)squeezing the dispersion solution through a polycarbonate membrane to obtain the xanthophyll liposome. The xanthophyll liposome modified by alpha-glucosyl hesperidin has high stability and long-term circulation performance, and significantly improves the absorption of xanthophyll in the eye.

Description

[0001] 1. Technical field [0002] The invention belongs to the technical field of functional food processing, and in particular relates to a preparation method of lutein liposome modified by alpha-glucosyl hesperidin. [0003] 2. Background technology [0004] Lutein is a natural carotenoid widely found in vegetables, fruits, flowers and some algae. The human body cannot synthesize it by itself and must be ingested or supplemented in the diet. Lutein is one of the main components of macular pigment in the eyes. It has the function of absorbing blue light and enhancing retinal tissue. It has significant effects in protecting vision and preventing cataracts. However, the molecular structure of lutein has a long carbon chain and contains multiple hydrophobic groups, which makes its solubility in water poor, which limits its application in food, medicine and other fields. [0005] Embedding lutein in the form of microcapsule technology, nano-self-emulsifying carrier, nano-emulsif...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/28A61K47/24A61K47/26A61K31/047A61P27/02
CPCA61K9/1277A61K47/28A61K47/24A61K47/26A61K31/047A61P27/02
Inventor 张钟元李大婧刘春泉聂梅梅江宁刘春菊牛丽影
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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