Hydrophobic deep eutectic solvent for removing ginkgolic acid in ginkgo leaf crude extract through liquid-liquid micro-extraction as well as preparation method thereof and removal method

Abstract

The invention discloses a hydrophobic deep eutectic solvent for removing ginkgolic acid in a ginkgo leaf crude extract through liquid-liquid micro-extraction as well as a preparation method thereof and a removal method. The hydrophobic deep eutectic solvent is prepared from menthol, hexyl alcohol and lauric acid according to a molar ratio of 1: 0.9: 0.1 to 1: 0.4: 0.6. The hydrophobic deep eutectic solvent is simple and rapid to prepare. The hydrogen bonds are stable, the fluidity is good, and the solvent is a green solvent and has the characteristics of easy biodegradation, no environmental pollution, and high biocompatibility. The hydrophobic deep eutectic solvent can be used in a method for removing ginkgolic acid in a ginkgo leaf crude extract through liquid-liquid micro-extraction, the content of ginkgolic acid in the ginkgo leaf crude extract can be reduced to 5ppm or less; meanwhile, the content of functional components such as gingko flavonoid and terpene lactones in the gingkoleaf crude extract is maintained, and the whole liquid-liquid micro-extraction removal method is simple, convenient, quick and safe to operate, high in removal efficiency, low in cost and free of harm to the environment and human bodies.

Description

technical field [0001] The invention relates to the processing and utilization of agricultural and forestry biological resources, in particular to a hydrophobic deep eutectic solvent used for liquid-liquid micro-extraction to remove ginkgolic acid in ginkgo biloba crude extract, and a preparation method and removal method thereof. Background technique [0002] Ginkgo biloba L. is a deciduous tree of the genus Ginkgo in the family Ginkgoaceae, and there is only one family and one genus in the Ginkgoles. The appearance of Ginkgo biloba can be traced back to hundreds of millions of years ago. It is the oldest relict plant among the gymnosperms left over from the Quaternary glacial movement, and is called a "living fossil". Ginkgo biloba is a multi-purpose tree species unique to my country for medicine, food, material and ornamental, and it is a rare plant under the second-class protection of the country. my country is the origin of ginkgo, and it is also the largest ginkgo pla...

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Problems solved by technology

[0005] Purpose of the invention: for the defects existing in the prior art, the present invention provides a new hydrophobic deep eutectic solvent for liquid-liquid microextraction to remove ginkgolic acid in the crude extract of ginkgo biloba, and the hydrophobic deep eutectic solvent can Efficient and simple method for realizing the removal of ginkgophenolic acid, while retaining the content of ginkgo flavonoids and terpene lactones to a large extent; it solves the problem of large amount of organic solvent usage and complicated operation in the existing method of removing ginkgolic acid , high cost, low efficiency and a series of problems

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