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Efficient composite mildew-proof preservative and application thereof

A preservative and high-efficiency technology, applied in the direction of application, biocide, fungicide, etc., can solve the problems of poor antibacterial effect, achieve the effect of no secondary pollution, reduce economic loss, scientific and safe ingredients

Inactive Publication Date: 2020-07-10
SHENZHEN INST OF GUANGDONG OCEAN UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both benzoic acid and sorbic acid are acidic preservatives, and their antibacterial effect is very poor in products with pH>5.5

Method used

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  • Efficient composite mildew-proof preservative and application thereof
  • Efficient composite mildew-proof preservative and application thereof
  • Efficient composite mildew-proof preservative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of high-efficiency composite anti-mildew preservative

[0034] The high-efficiency composite anti-mildew preservative provided by the present invention is mainly composed of the following components: calcium propionate 100-10000PPM, bromogeramine 100-10000PPM, natamycin 100-10000PPM, paraben 100-10000PPM, and the balance is water.

[0035] According to the preparation of the above components, a high-efficiency composite anti-mildew preservative can be obtained.

Embodiment 2

[0036] Example 2: Anti-mildew effect detection of high-efficiency composite anti-mildew preservative

[0037] 1) Selected strains for testing: Aspergillus niger, Aspergillus oryzae, Trichoderma, Penicillium, Aspergillus white, and Aspergillus flavus (all are molds isolated from biological specimens);

[0038] 2) Detection method: plate inhibition zone method (using nutrient agar plate);

[0039] 3) Detection temperature: 26℃±2℃;

[0040] 4) Detection time: 72 hours;

[0041] 5) test result: antiseptic preparation of the present invention all has obvious inhibitory effect to selected bacterial classification, and diameter of inhibition zone is as follows: Aspergillus albicans 185mm, Aspergillus niger 125mm, Aspergillus flavus 130mm, Penicillium 125mm, Trichoderma 98mm, rice Aspergillus 135mm.

Embodiment 3

[0042] Embodiment 3: Bacteriostatic effect detection of high-efficiency compound anti-mildew preservative

[0043] 1) Selected test strains: Bacillus subtilis, Escherichia coli, Proteus, Streptococcus, Staphylococcus aureus, etc. (all are routine laboratory strains);

[0044] 2) Detection method: plate inhibition zone method (using nutrient agar plate);

[0045] 3) Detection temperature: 35°C±1°C;

[0046] 4) Detection time: 24 hours;

[0047] 5) Test result: measure with a caliper. Experimental results show that the antiseptic preparation of the present invention has obvious inhibitory effects on the selected strains, and the diameter of the inhibition zone is between 8-33mm.

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Abstract

The invention provides an efficient composite mildew-proof preservative and an application thereof, and belongs to the technical field of marine organism mildew prevention and corrosion prevention. The efficient composite mildew-proof preservative provided by the invention is mainly prepared from the following components in concentration content: 100-10,000 PPM of calcium propionate, 100-10,000 PPM of bromogeramine, 100-10,000 PPM of natamycin, 100-10,000 PPM of nipagin ester and the balance of water. The composite mildew-proof preservative is composed of environment-friendly, odorless, colorless and low-toxicity components, effectively reduces damage of volatile smell to human bodies, has little harm to the human bodies and the environment, is easy to manufacture and simple and convenientin method, has good preservation, fixation, sterilization, mildew-proof and preservative effects on specimens, has great popularization value, and is an ideal substitute product of formalin preservation liquid.

Description

technical field [0001] The invention relates to the technical field of anti-mildew and anti-corrosion of marine organisms, in particular to a high-efficiency composite anti-mold anti-corrosion agent and its application. Background technique [0002] There are many kinds of marine organisms, and the methods of making and preserving different types of biological specimens are also different. With the deepening of marine biological research, the collection and preservation of marine biological specimens is of great significance. At the same time, marine biological specimens, as an important material for biology, are an important model for teaching and an important carrier for scientific communication. Therefore, making marine organisms into specimens has important teaching and scientific significance. [0003] Specimens are easily damaged by mold hazards, resulting in shortened service life and economic losses. Therefore, anti-mold and anti-corrosion is a key part of specimen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01N37/40A01N37/02A61K31/14A01P3/00A01N1/00
CPCA01N1/00A01N37/02A01N37/40A01N43/90A61K31/14
Inventor 张锐孙美榕杜慧廖艳
Owner SHENZHEN INST OF GUANGDONG OCEAN UNIV