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Application of porous nanomaterials in regulating the pluripotency of embryonic stem cells

An embryonic stem cell and nanomaterial technology, applied in the field of stem cell pluripotency regulation, can solve problems such as poor stability and high cost

Active Publication Date: 2021-11-19
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problems of poor stability and high cost of traditional embryonic stem cell pluripotency regulators, creatively screens and designs porous nanomaterials as regulators, and utilizes its excellent properties such as easy modification, high stability, high specific surface area and porosity. characteristics, preparation of new embryonic stem cell pluripotency regulatory preparations to overcome the limitations of traditional materials in the field of embryonic stem cells

Method used

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  • Application of porous nanomaterials in regulating the pluripotency of embryonic stem cells
  • Application of porous nanomaterials in regulating the pluripotency of embryonic stem cells
  • Application of porous nanomaterials in regulating the pluripotency of embryonic stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of VMOP-2.

[0032] Weigh 0.03g of vanadium chloride and 0.02g of 2-aminoterephthalic acid into a 20mL high-temperature reaction kettle, and add 2mL of N,N-dimethylformamide and 0.5mL of absolute ethanol into it. The system was placed in an oven and heated at 150°C for 48 hours. After the reaction, the reaction mixture was centrifuged, and the supernatant was discarded to obtain a yellow-green solid, which was washed several times with absolute ethanol. Put it in a desiccator and dry at room temperature for 24 hours. figure 1 : Powder diffraction data show that the synthetic VMOP-2 is consistent with its structure simulation results, indicating that VMOP-2 is prepared successfully. figure 2 : The results of scanning electron microscopy showed that the VMOP-2 crystal is a regular octahedral structure.

[0033] Preparation of VMOP-1.

[0034] Weigh 0.03g of vanadium chloride and 0.02g of 2-terephthalic acid into a 20mL high-temperature reactor, and add 2m...

Embodiment 2

[0039] Dissolve 1 mg of VMOP-2 material in 1 mL of PBS solution, take it out at 1 hour, 1 day, 2 days, 3 days, and 4 days respectively, and use a UV spectrophotometer to scan the VMOP-2 solution at full wavelength (200-800nm) . The result is as image 3 As shown, the UV spectrum of VMOP-2 remains consistent until 4 days, which proves that the material has good stability in the solution state.

Embodiment 3

[0041] Regulation of VMOP-2 on self-renewal of mouse embryonic stem cells

[0042](1) Alkaline phosphatase staining detects the regulation of the self-renewal of stem cells by the nanomaterials of the present invention: the embryonic stem cells in the exponential phase are spread in a six-well plate, and after the cells adhere to the wall, the traditional pluripotent stem cells in the original medium of the experimental group are The pluripotent preparation Lif was replaced with VMOP-2, and the original medium containing the pluripotent preparation Lif was replaced with a medium not containing Lif as a control group, and placed in CO 2 Cultivate in a constant temperature incubator for 48 hours. Aspirate the supernatant, fix with 4% paraformaldehyde, and wash with buffer 1-2 times. Prepare 3.03 mL of BCIP / NBT staining working solution, the specific method is as follows: add 10 μL of BCIP solution (300×) and 20 μL of NBT solution (150×) to 3 mL of alkaline phosphatase chromogen...

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Abstract

Aiming at the problems of high price and poor stability of the traditional leukemia inhibitory factor (LIF) required for maintaining the pluripotency of embryonic stem cells, the present invention creatively uses porous nanomaterials to regulate the pluripotency of mouse embryonic stem cells. Porous nanomaterials (organic porous nanomaterials and inorganic porous nanomaterials) can regulate the pluripotency of embryonic stem cells under the culture condition without adding leukemia inhibitory factor (LIF). Porous nanomaterials have the advantages of good biocompatibility, cost saving, and good storage, which will better promote the basic research and application of embryonic stem cells.

Description

technical field [0001] The invention belongs to the field of stem cell pluripotency regulation, and in particular relates to the application of porous nanomaterials in regulating the pluripotency of embryonic stem cells. Background technique [0002] Embryonic stem cells are cells derived from the inner cell mass (ICM) of blastocysts, which have two characteristics of self-renewal and differentiation. Self-renewal is the ability of cells to proliferate in the same state, and differentiation is the ability to develop into all cell lineages. In the aging or pathological state of the human body, stem cells can directly stimulate tissue regeneration or recruit cytokines to promote tissue repair through differentiation into various types of cells, or through paracrine effects. Based on the above characteristics, research on stem cells in the field of clinical medicine has developed rapidly. Stem cell clinical trials involve spinal cord injury, multiple sclerosis, stroke, amyotro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0735
CPCC12N5/0606C12N2501/235C12N2501/999
Inventor 陈瑶薛雪安红德王冉
Owner NANKAI UNIV
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