Lactobacillus plantarum with inhibition effect on ETEC, and fermentation product and application thereof

A technology of Lactobacillus plantarum and fermentation products, applied in the directions of bacteria, lactobacillus, and application used in food preparation, can solve the problems of restricting the use of antibiotics, antibiotic residues, serious problems, etc., and achieve good acid stability and acid-base tolerance The effect of reducing the ratio of feed to meat and promoting animal growth

Active Publication Date: 2020-08-04
泰安肽普德蛋白有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Antibiotics are still the most widely used to treat piglet diarrhea at present, but the problem of antibiotic residues is becoming more and more serious, and the development and spread of antibiotic-resistant strains also limit the use of antibiotics

Method used

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  • Lactobacillus plantarum with inhibition effect on ETEC, and fermentation product and application thereof
  • Lactobacillus plantarum with inhibition effect on ETEC, and fermentation product and application thereof
  • Lactobacillus plantarum with inhibition effect on ETEC, and fermentation product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation and Identification of Lactobacillus plantarum (Lactobacillus plantarum) DMSLP0315

[0045] 1. 164 strains of lactic acid bacteria isolated from pig manure and 168 strains of lactic acid bacteria preserved in the laboratory of Acheng Sanhua Pig Breeding Base of Northeast Agricultural University.

[0046] After 85 pig feces samples were serially diluted and cultured, 164 suspected lactic acid bacteria strains with calcium-dissolving circles were selected and named after the letter H. After culturing the isolated strains on MRS solid medium for 48 hours, it was observed that the colonies were mostly milky white Round, slightly raised in the middle, opaque or translucent, with neat edges. Gram staining was positive, and the shape of the bacteria was short rod, long rod or spherical. Inoculate the Lactobacillus and Lactococcus frozen in the refrigerator at -20°C with 2% inoculum in MRS and M17 liquid medium respectively. Cultivate at 37°C for 16 hours, a...

Embodiment 2

[0072] The influence of embodiment 2 culture conditions on plantaractobacillus DMSLP1210 antibacterial activity

[0073] (1) Effect of culture time on the antibacterial activity of the target strain

[0074] Inoculate the screened target strain DMSLP1210 in the MRS liquid medium at an inoculum size of 1%, culture it statically at 37°C for 32 hours, and take samples every two hours starting from 16 hours, take 10mL to prepare the cell-free supernatant, and use ETEC CVCC 1518 was used to measure the antibacterial activity of the indicator bacteria and determine the optimum culture time. The influence of culture time on the antibacterial activity of Lactobacillus plantarum DMSLP1210 see Figure 5 . Depend on Figure 5 It can be seen that with the increase of culture time from 16-24h, the antibacterial activity of the strains gradually increased, the antibacterial activity reached the maximum at 24h, and the antibacterial activity of the strains tended to be stable at 26h-30h. ...

Embodiment 3

[0077] Example 3 Safety Evaluation of Lactobacillus plantarum DMSLP1210

[0078] 1. Hemolytic test

[0079] Streptococcus pyogenes was used as a control to conduct a hemolytic test. After Streptococcus pyogenes was inoculated on Columbia blood agar plate, a transparent hemolytic zone appeared around the colony, which is type B hemolysis (β hemolysis); after Lactobacillus plantarum DMSLP0315 and Lactobacillus plantarum DMSLP1210 were inoculated on Columbia blood agar plate, observe the colonies There is no hemolytic circle around, so it can be judged that the two strains are not hemolytic, and will not cause hemolytic hazard.

[0080] 2. Sensitivity to antibiotics

[0081] According to the evaluation criteria of the American Clinical and Laboratory Standards Institute CLSI, the susceptibility results (Table 2) of the two strains to 7 commonly used antibiotics were obtained. Resistant to vancomycin and sensitive to tetracycline and ampicillin. Among them, Lactobacillus plant...

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Abstract

The invention provides lactobacillus plantarum with an inhibiting effect on ETEC as well as a fermentation product and application of the lactobacillus plantarum, and belongs to the technical field ofpreparation of probiotics and feed additives. The lactobacillus plantarum (Lactobacillus plantarum) provided by the invention is named as DMS LP1210, and the preservation number is GDMCC No.60717. The bacterium has the function of inhibiting ETEC, the bacteriostatic activity of the produced bacteriocin is still kept at 80% or above after the bacteriocin is subjected to heat treatment at 121 DEG Cfor 30 min, and the bacteriocin has tolerance to high temperature; 80% or above of the antibacterial activity is reserved when the pH value is 2-10, 90% or above of the antibacterial activity is reserved after pepsin, trypsin and protease K are used for treating the bacteria, the tolerance to the intestinal tract environment is good, the diarrhea rate is reduced, the feed conversion efficiency isimproved, the effect is safe and reliable, and the probiotics additive is suitable for being developed into feed probiotics additives.

Description

technical field [0001] The invention relates to the technical field of preparation of probiotics and feed additives, in particular to plantaractobacillus having an inhibitory effect on ETEC and its fermentation product and application. Background technique [0002] Escherichia coli, commonly referred to as E. coli, belongs to the genus Escherichia and is a Gram-negative bacterium. Most E. coli strains that live in the gut are harmless, however, there are some pathogenic strains that can cause diarrheal disease in piglets. According to different biological properties, pathogenic Escherichia coli can be divided into six categories: enteroinvasive Escherichia coli (EIEC), enteropathogenic Escherichia coli (Enteropathogenic Escherichia coli, EPEC), enterohaemorrhagic Escherichia coli ( Enterohemorrhagic Escherichia coli (EHEC), Enterotoxigenic Escherichia coli (ETEC), Enteroadherent Escherichia coli (EAEC), Diffuselyadherent Escherichia coli (DAEC). At present, enterotoxigenic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04C12R1/25
CPCC12N1/20A23K10/18A61K35/747A61P31/04C12R2001/25C12N1/205A23V2400/169Y02A50/30
Inventor 申光荣孟祥晨
Owner 泰安肽普德蛋白有限公司
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