A method for establishing the fingerprint of Baoyuan decoction preparation
A technique of fingerprinting and establishing methods, which is applied in the field of drug detection, can solve problems such as difficulty in fully reflecting the comprehensive information of Baoyuan Decoction, complicated ingredients, and many flavors of Baoyuan Decoction
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Embodiment 1
[0065] 1. Solution preparation
[0066] 1.1 Preparation of the test solution
[0067] S1, mix 3.75g ginseng, 7.5g astragalus, 1.85g licorice, and 0.75g cinnamon, add 10 times the amount of water, soak in a ceramic pot for 30min, bring to a boil over high heat, keep boiling on low heat, continue to cook for 40min, and filter while hot , concentrate under reduced pressure at 60°C, and dilute to 25mL with water to obtain a decoction;
[0068] S2. Accurately measure 1 mL of the decoction and add it to a measuring bottle, add 6 times the volume of absolute ethanol, vortex for 3 min, centrifuge at 12000 rpm for 10 min, evaporate the supernatant to dryness, and dilute it to a 2 mL volumetric flask with methanol. Continue the filtrate to obtain the test solution.
[0069] 1.2 Preparation of mixed reference solution
[0070] Weigh ginsenoside Rg 1 , Ginsenoside Re, Ginsenoside Rb 1 , acteosin glucoside, acteosin, formononetin, formononetin, liquiritin, glycyrrhizinic acid monoammo...
Embodiment 2
[0091] Methodology Validation:
[0092] Precision, repeatability and stability tests
[0093] Precision: Take the test solution prepared under item 1.1, measure it according to the above high-performance liquid chromatography conditions, and inject 6 times continuously. In the UPLC-DAD / ELSD fingerprint at 260nm, the chromatographic peak at the retention time of 31.32min is used as the reference peak , under ELSD, the chromatographic peak at the retention time of 31.42min was used as the reference peak, and the precision was evaluated by the relative peak area of each main chromatographic peak and the RSD value of the relative retention time.
[0094] Repeatability: Take the same batch of Baoyuantang test solution, prepare 6 parts of test solution in parallel according to the preparation method of the test solution under item 1.1, measure according to the above high performance liquid chromatography conditions, UPLC-DAD / ELSD fingerprint In the spectrum, the chromatographic p...
Embodiment 3
[0104] 3.1 Determination of common peaks:
[0105] Take 16 batches of Baoyuan Decoction raw materials, and combine each herbal decoction piece in the formula into 16 batches according to the principle of random combination according to different batches (denoted as S1-S16), of which 1-13 batches are randomly combined by using SPSS software, 14 and 15 batches are then based on the assay results of each decoction piece (Chinese Pharmacopoeia test method), the batch combination with the highest content determination of each medicinal herb piece is the 14th batch, and the lowest batch combination is the 15th batch; The same amount of each batch of single herbal decoction pieces was mixed, and then the mixed single herbal decoction pieces were combined into the 16th batch. The combination is shown in Table 4.
[0106] Table 4 Random combination of single-flavor decoction pieces
[0107]
[0108] According to embodiment 1, the preparation operation of need testing solution under...
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