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Method for inducing propagation of stem buds by leaf callus of burretiodendron esquirolii

A callus induction and callus technology is applied in the field of plant tissue culture, which can solve the problems of differentiation, can not maximize the genetic improvement gain, and has no stalk pterosaur tissue culture technology, and achieves a short cycle and promotes popularization. Planting, low cost effect

Inactive Publication Date: 2020-08-07
YULIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, sexual reproduction suffers from the problem of differentiation and cannot maximize genetic improvement gain, while tissue culture can ensure relative uniformity of offspring and increase genetic gain
[0006] At present, there is no relevant research on the tissue culture technology of samara at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Collection of explants: collecting annual samara branches with a diameter of 5-10 mm and a length of about 15 cm in good physiological state and without pests and diseases with scissors, and taking leaves from the collected branches as explants.

[0033] (2) Leaf callus induction culture: select the tender and unscarred leaflets from the leaves collected in step (1) back to the laboratory, wash them with tap water for 3 hours, place them in a 70% ethanol solution in an ultra-clean workbench Sterilize twice in medium, 20 seconds each time, and then use 10% H 2 o 2 (Which drops 2 drops of Tween-20) Stir and sterilize twice for 3 minutes each time, and finally rinse with sterile water for 5-7 times until it is completely rinsed. Cut the sterilized samara leaves into small pieces of 5 mm × 5 mm, and then inoculate them horizontally in the leaf callus induction medium with the leaf surface facing up, and cultivate for 30 days to induce the formation of callus, and the i...

Embodiment 2

[0042] (1) Collection of explants: collecting annual samara branches with a diameter of 5-10 mm and a length of about 15 cm in good physiological state and without pests and diseases with scissors, and taking leaves from the collected branches as explants.

[0043] (2) Leaf callus induction culture: select the tender and unscarred leaflets from the leaves collected in step (1) back to the laboratory, wash them with tap water for 3 hours, place them in a 70% ethanol solution in an ultra-clean workbench Sterilize twice in medium, 20 seconds each time, and then use 10% H 2 o 2 (Which drops 2 drops of Tween-20) Stir and sterilize twice for 3 minutes each time, and finally rinse with sterile water for 5-7 times until it is completely rinsed. Cut the sterilized samara leaves into small pieces of 5 mm × 5 mm, and then inoculate them horizontally in the leaf callus induction medium with the leaf surface facing upwards. After culturing for 35 days, the callus can be induced to form, a...

Embodiment 3

[0052] (1) Collection of explants: collecting annual samara branches with a diameter of 5-10 mm and a length of about 15 cm in good physiological state and without pests and diseases with scissors, and taking leaves from the collected branches as explants.

[0053] (2) Leaf callus induction culture: select the tender and unscarred leaflets from the leaves collected in step (1) back to the laboratory, wash them with tap water for 3 hours, place them in a 70% ethanol solution in an ultra-clean workbench Sterilize twice in medium, 20 seconds each time, and then use 10% H 2 o 2 (Which drops 2 drops of Tween-20) Stir and sterilize twice for 3 minutes each time, and finally rinse with sterile water for 5-7 times until it is completely rinsed. Cut the sterilized samara leaves into small pieces of 5 mm × 5 mm, then inoculate them horizontally in the leaf callus induction medium with the leaf surface facing up, and cultivate for 40 days to induce the formation of callus, and the induc...

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Abstract

The invention discloses a method for inducing propagation of stem buds by leaf callus of burretiodendron esquirolii and belongs to the field of plant tissue cultivation. The method comprises steps asfollows: leaves on branches are collected as explants; leaves are cut and induced to form callus after being disinfected; adventitious bud induction culture is performed; rooting culture is performed;and transplantation is performed. The method for inducing propagation of stem buds by leaf callus of burretiodendron esquirolii is established with a plant tissue culture technology, the leaf callusinduction rate reaches 73% or above, and the transplantation survival rate reaches 83% or above. The method has the characteristics of short cycle, low cost, high propagation coefficient, consistent seedling growth and the like, can be directly used for industrial production of seedlings of the burretiodendron esquirolii and has great significance in promotion of popularization and planting of theburretiodendron esquirolii.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inducing stem bud propagation by callus of leaves of samara. Background technique [0002] Samara, scientific name: Burretiodendron esquirolii (Levl.) Rehd., a endangered species, belongs to the national second-level key protected wild plants. Due to deforestation, it is now only scattered in secondary forests, and it is difficult to find natural forests dominated by samara. [0003] In the natural forest, the samara is often the upper layer of the stand; in the sparse forest, there are many young trees in the forest, and when they form a larger community with other plants, they have greater stability; in the Luodianyangli area, the stalk Samara often forms a forest stand with carton bamboo, white wax tree, camel tree, neem tree, emblica, stone rock maple, hangzi shoot, and fake tobacco leaf tree. [0004] A large number of introduction experiments and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 莫昭展黄远抗卢娟
Owner YULIN NORMAL UNIVERSITY