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Preparation method of phage-CuNPs @ MWCNTs

A phage and carboxylation technology, which is applied in the field of preparation of phage-CuNPs@MWCNTs, can solve the problems of no targeting and reduce the application direction of Cu nanoparticles, and achieve the effect of improving safety and targeting bacteria well

Inactive Publication Date: 2020-08-14
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cu nanoparticles are a widely used photothermal agent, but they have no targeting, which greatly reduces the application direction of Cu nanoparticles.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] The preparation method of phage-CuNPs@MWCNTs includes the following steps:

[0014] (1) Carboxylation of MWNTs: Add 2.0g of MWNTs and 100ml of concentrated nitric acid:dilute sulfuric acid (1:3) into a 500ml flask, and after ultrasonic dispersion, sonicate in an oil bath at 50°C for 6 hours. Dilute with 5 times the amount of water, centrifuge, filter with suction (0.22 aqueous filter membrane), and wash with water to neutral. Placed in an oven at 80℃ and dried for 12h to obtain MWCNTs samples.

[0015] (2) Synthesis of CuNPs@MWCNTs: Weigh 3.0mg carboxylated MWCNTs into a 500ml flask, sonicate for 0.5h and disperse in 3mL ultrapure water, add 4.6mmol / L CuSO 4 300μL of the solution was stirred for 4h. After the suspension was uniformly sonicated, 11.2mg of AA solution (2mL) was added dropwise, and heated and stirred in a water bath at 80℃ for 12h. After cooling, ultrasonic dispersion is uniform, and centrifugal washing with deionized water 2 times. The finally obtained mate...

Embodiment 2

[0018] The preparation method of phage-CuNPs@MWCNTs includes the following steps:

[0019] (1) Carboxylation of MWNTs: Add 2.0g of MWNTs and 100ml of concentrated nitric acid:dilute sulfuric acid (1:3) into a 500ml flask, and after ultrasonic dispersion, sonicate in an oil bath at 50°C for 6 hours. Dilute with 5 times the amount of water, centrifuge, filter with suction (0.22 aqueous filter membrane), and wash with water to neutral. Placed in an oven at 80℃ and dried for 12h to obtain MWCNTs samples.

[0020] (2) Synthesis of CuNPs@MWCNTs: Weigh 3.0mg carboxylated MWCNTs into a 500ml flask, disperse in 3mL ultrapure water with ultrasound for 0.5h, add 300μL of 4.6mmol / L CuSO4 solution, and stir for 4h. After the suspension was uniformly sonicated, 11.2mg of AA solution (2mL) was added dropwise, and heated and stirred in a water bath at 80℃ for 12h. After cooling, ultrasonic dispersion is uniform, and centrifugal washing with deionized water 2 times. The finally obtained material...

Embodiment 3

[0023] The preparation method of phage-CuNPs@MWCNTs includes the following steps:

[0024] (1) Carboxylation of MWNTs: Add 2.0g of MWNTs and 100ml of concentrated nitric acid: concentrated sulfuric acid (1:3) into a 500ml flask, and after ultrasonic dispersion, sonicate in an oil bath at 50°C for 6 hours. Dilute with 5 times the amount of water, centrifuge, filter with suction (0.22 aqueous filter membrane), and wash with water to neutral. Placed in an oven at 80℃ and dried for 12h to obtain MWCNTs samples.

[0025] (2) Synthesis of CuNPs@MWCNTs: Weigh 3.0mg carboxylated MWCNTs into a 500ml flask, disperse in 3mL ultrapure water with ultrasound for 0.5h, add 300μL of 4.6mmol / L CuSO4 solution, and stir for 4h. After the suspension was uniformly sonicated, 11.2mg of AA solution (2mL) was added dropwise, and heated and stirred in a water bath at 80℃ for 12h. After cooling, ultrasonic dispersion is uniform, and centrifugal washing with deionized water 2 times. The finally obtained m...

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PUM

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Abstract

The invention discloses a preparation method of phage-CuNPs @ MWCNTs. The preparation method comprises the following steps: (1) carboxylation of MWNTs; (2) synthesis of CuNPs @ MWCNTs; and (3) preparation of the phage-CuNPs @ MWCNTs. The phage-CuNPs @ MWCNTs have triple functions: 1) the phage can efficiently target bacteria; 2) CuNPs are brought to a bacterial aggregation position under the targeting of the phage, and CuNPs light-heat can kill bacteria; and 3) the CuNPs light-heat not only can kill bacteria, but also can kill the phage, so that the safety of killing bacteria by the phage is greatly improved.

Description

Technical field [0001] The present invention relates to a preparation method of bacteriophage-CuNPs@MWCNTs. The carboxylated Cu nanoparticles can be combined with bacteriophages. The bacteriophages have the function of targeting bacteria. Therefore, Cu also has a targeting function, which provides a novel target for bacterial targeting. Research methods. Background technique [0002] Bacteriophage is a kind of virus. Bacteriophage has some characteristics of virus: the individual is small; it does not have a complete cell structure; it only contains a single nucleic acid. Can be regarded as a kind of "predator" bacteria. The phage genome contains many genes, but all known phages use bacterial ribosomes, various factors required for protein synthesis, various amino acids and energy production systems to achieve their own growth and proliferation in bacterial cells. Once left the host cell, the phage can neither grow nor replicate. Bacteriophages are a ubiquitous organism and ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/51A61P31/04
CPCA61K41/0052A61K47/51A61P31/04
Inventor 郑斌郭明明明东甘霖
Owner TIANJIN UNIV