Indel marker and method for identifying purity of cucumber hybrid seeds

Abstract

The invention discloses an Indel marker and a method for identifying the purity of cucumber hybrid seeds. The marker comprises an Indel 79 forward primer Indel 79-F and a reverse primer Indel 79-R, wherein wherein the nucleotide sequence of the forward primer Indel 79-F is as shown in SEQ ID NO.1, and the nucleotide sequence of the reverse primer Indel 79-R is as shown in SEQ ID NO.2. The invention further discloses a method for identifying the purity of the cucumber hybrid seeds. The method is used for identifying the purity of the cucumber Lvmei No.1 hybrid seeds, 18C686 hybrid seeds, 19C147hybrid seeds and 19C087 hybrid seeds. The purity of the four cucumber hybrid seeds, namely the cucumber Lvmei No.1, 18C686, 19C147 and 19C087 can be effectively identified only by simply extracting the genome DNA of the cucumber leaf and then carrying out PCR amplification and agarose gel electrophoresis. The method can replace a field purity identification method, greatly shortens the identification period, saves the identification cost, improves the identification accuracy, and has strong commercial application value.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to an Indel for purity identification of cucumber hybrid seeds [0002] notation and method. Background technique [0003] Cucumber is one of the vegetables with the largest planting area in my country, and it is also one of the main economic crops in China. At present, with the gradual expansion of the cultivation area of ​​protected areas and glass greenhouses, the cultivation of cucumbers has also changed from one crop a year to three crops a year. Cucumber varieties are also gradually enriched, and they no longer exist as a single vegetable. Since different regions have different demands for cucumber varieties, breeders have to select and breed different types of cucumbers according to regions. At the same time, seed quality is also quite important, and seed purity is one of the important indicators to measure seed quality. [0004] At present, the main method of c...

AI Technical Summary

Problems solved by technology

In the process of cucumber hybrid breeding, due to reasons such as pollen mixing and insect pollination, false hybrids often appear, resulting in a decrease in seed purity and seed quality, causing huge economic losses in production and sales

The traditional purity identification is mainly morphological identification through field regional tests. Although the identification results are relatively accurate, the determination of plant morphology takes a long time, which is difficult to meet the needs of rapid determination during the investigation process. Big

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