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Preparation method of sirolimus molecularly imprinted erbium nitride composite paste electrode

A technology of molecular imprinting and erbium nitride, which is applied in the fields of analyzing materials, electrochemical variables of materials, and nanotechnology for sensing, etc., can solve problems such as mutual interference of coexisting substances, low sirolimus content, and accuracy limitations. , to achieve the effect of high affinity and selectivity, simple preparation process and high sensitivity

Active Publication Date: 2020-08-18
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for detecting sirolimus mainly include high-performance liquid chromatography. The accuracy of these methods is limited, and the equipment is relatively expensive and requires professional operation. The long detection cycle also limits its application.
In addition, due to the relatively low content of sirolimus in biological samples and the mutual interference of coexisting substances, it is difficult to accurately detect sirolimus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Preparation of erbium nitride composite paste electrode: In an agate mortar, add nanometer erbium nitride: 50g, carbon oxide nanotubes: 14g, N-sulfonic acid butylpyridinium bisulfate: 12g, selenide Cadmium: 10g, liquid paraffin: 8g, n-heptane: 9 mL, grind evenly to obtain the carbon paste of the mixture; then put the carbon paste into a glass tube with an inner diameter of Φ5.5mm connected with a wire, compact it, and dry it. Grinding with polishing powder, polishing, and washing with deionized water, the erbium nitride composite paste electrode is obtained;

[0023] (2) Preparation of vinyltriacetoxysilane-modified erbium nitride composite paste electrode: In the reactor, add ethanol: 94 mL, vinyltriacetoxysilane: 26 g, stir and dissolve, and polish the Put the gallium nitride paste electrode in, soak it at room temperature for 6 hours, raise the temperature to 60±2°C and react at a constant temperature for 2 hours, take out the electrode, wash it with absolute eth...

Embodiment 2

[0027](1) Preparation of erbium nitride composite paste electrode: In an agate mortar, add nano-erbium nitride: 24g, carbon oxide nanotubes: 6g, N-sulfonic acid butylpyridinium bisulfate: 5g, selenide Cadmium: 6g, liquid paraffin: 5g, n-heptane: 6mL, grind evenly to obtain the carbon paste of the mixture; then put the carbon paste into a glass tube with an inner diameter of Φ5.5mm connected with a wire, compact, dry, and use Grinding with polishing powder, polishing, and washing with deionized water to obtain the erbium nitride composite paste electrode;

[0028] (2) Preparation of vinyltriacetoxysilane-modified erbium nitride composite paste electrode: In the reactor, add ethanol: 91 mL, vinyltriacetoxysilane: 28g, stir and dissolve, and polish Put the gallium nitride paste electrode in, soak it at room temperature for 6 hours, raise the temperature to 60±2°C and react at a constant temperature for 2 hours, take out the electrode, wash it with absolute ethanol, and dry it to ...

Embodiment 3

[0032] (1) Preparation of erbium nitride composite paste electrode: In an agate mortar, add nanometer erbium nitride: 26g, carbon oxide nanotubes: 6.5g, N-sulfonic acid butylpyridinium bisulfate: 7g, selenium Cadmium chloride: 4g, liquid paraffin: 3g, n-heptane: 5mL, grind evenly to get the carbon paste of the mixture; then put the carbon paste into a glass tube with an inner diameter of Φ5.5mm connected with a wire, compact it, and dry it. Grinding with polishing powder, polishing, and washing with deionized water, the erbium nitride composite paste electrode is obtained;

[0033] (2) Preparation of vinyltriacetoxysilane-modified erbium nitride composite paste electrode: In the reactor, add ethanol: 96 mL, vinyltriacetoxysilane: 24 g, stir and dissolve, and polish the Put the gallium nitride paste electrode in, soak it at room temperature for 6 hours, raise the temperature to 60±2°C and react at a constant temperature for 2 hours, take out the electrode, wash it with absolute...

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Abstract

The invention discloses a preparation method of a sirolimus molecularly imprinted erbium nitride composite paste electrode. The method comprises the steps: preparing erbium nitride composite paste electrode from nano erbium nitride, carbon oxide nanotubes, cadmium selenide and N-sulfobutyl pyridine bisulfate; modifying the erbium nitride composite paste electrode by adopting vinyltriacetoxysilaneto prepare a vinyltriacetoxysilane modified erbium nitride composite paste electrode; preparing a sirolimus molecularly imprinted polymer by taking template molecules, acrylamide and 3-aminopyrrole asmonomers and ethylene glycol dimethacrylate as a cross-linking agent and dimethyl azodiisobutyrate as an initiator; preparing the sirolimus molecularly imprinted erbium nitride composite paste electrode sensor by modifying a vinyl triacetoxysilane modified erbium nitride composite paste electrode with the sirolimus molecularly imprinted polymer by adopting a dispensing method. The sensor has theadvantages of high sensitivity to sirolimus, good specificity and rapid detection, and can be repeatedly used.

Description

technical field [0001] The present invention relates to a preparation method of a molecularly imprinted sensor and the technical field of rapid detection application, in particular to a preparation method of a sirolimus molecularly imprinted erbium nitride compound paste electrode for detecting sirolimus in a sample . Background technique [0002] Sirolimus (also known as "rapamycin") is a new type of macrolide immunosuppressant, which is a white solid crystal with a melting point of 183-185°C and is lipophilic. It was developed as early as the 1970s. It was originally used as a low-toxic antifungal drug. It was found to have an immunosuppressive effect in 1977. It was used as a new drug for the treatment of organ transplant rejection in 1989. From animal experiments and According to the effect of clinical application, it is a new type of immunosuppressant with good curative effect, low toxicity and no nephrotoxicity. Now it is often used as a drug to maintain the immunity...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30B82Y15/00
CPCG01N27/48G01N27/30G01N27/308B82Y15/00
Inventor 李慧芝翟玉博李志英
Owner UNIV OF JINAN