Cabbage type rape gene editing material based on CRISPR/Cas9

Abstract

The invention discloses a cabbage type rape gene editing material based on CRISPR / Cas9. A method comprises the following steps: knocking out one or more than one of six copies (BnTFL1-1, BnTFL1-2, BnTFL1-3, BnTFL1-4, BnTFL1-5 and BnTFL1-6) of the cabbage type rape BnTFL1 gene by utilizing a CRISPR / Cas9 gene editing technology; so that the cabbage type rape gene edited material with advanced flowering time, terminal flowers on main branches and all side branches, and limited inflorescences changed from infinite inflorescences is obtained; different copies of edited gene editing materials show the same flowering time, floral organ and inflorescence structure phenotype, it indicates that the six copies of the cabbage type rape BnTFL1 gene all have the functions of regulating and controlling the flowering time, floral organ development and inflorescence structure, and no additive effect exists. The gene edited material can be used as rape materials or varieties suitable for mechanized harvesting, and precious gene resources and germplasm resources are provided for cabbage type rape breeding.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering and biotechnology, and relates to a method and application of site-directed mutation of Brassica napus BnTFL1 gene, in particular to a CRISPR / Cas9-based Brassica napus gene editing material. Background technique [0002] Rapeseed is one of the most important oil crops in the world today because of its high oil production efficiency, strong stress resistance and wide adaptability. As the largest source of domestic edible vegetable oil, rape accounts for more than 55% of the oil production of domestic oil crops. It is the core of maintaining the national edible oil supply security. Its planting area and output both rank first in the world. The main products of rapeseed are rapeseed oil and rapeseed meal. Rapeseed oil accounts for more than 50% of the total vegetable oil in my country's edible vegetable oil supply, and is rich in unsaturated fatty acids, vitamins and other nutrients needed b...

AI Technical Summary

Problems solved by technology

[0007] As an allotetraploid crop, Brassica napus has a complex genome and many homologous copies, and there are often gene redundancy and gene additive effects between different homologous copies

Using conventional breeding techniques to breed Brassica napus varieties with a short growth period, limited inflorescence and consistent flowering period often takes a long time, and is time-consuming and labor-intensive; there is a large blindness in mutation breeding, and the direction and nature of mutation cannot be controlled , less beneficial variation, improved quantitative traits are poor

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