Method for rapidly detecting ganoderic acid G in ganoderma lucidum spore oil

A technology of Ganoderma lucidum spore oil and detection method, which is applied in the field of rapid detection of Ganoderma lucidum acid G in Ganoderma lucidum spore oil, can solve problems such as low sensitivity, and achieve the effects of low cost, low cost and harm reduction.

Pending Publication Date: 2020-08-21
河北省食品检验研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surface-enhanced Raman spectroscopy (SERS) uses trace molecules to adsorb on Cu, Ag, Au and other metal sols and electrode surfaces, and its Raman spectrum signal can be enhanced by 10 4 ~10 6 , overcoming the shortcomings of low sensitivity of conventional Raman spectroscopy

Method used

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  • Method for rapidly detecting ganoderic acid G in ganoderma lucidum spore oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] The rapid qualitative analysis of ganoderma acid G in the ganoderma lucidum spore oil of embodiment 1

[0045] Sample: taken from the commercially available Ganoderma lucidum spore oil A.

[0046] a. Weigh 0.4 g of spore oil, add 2 mL of anhydrous methanol for oscillation, and place in an ultrasonic extractor for 10 minutes of sonication.

[0047] b. Shake the extract and filter.

[0048] Qualitative analysis:

[0049] Take 200 μL of filtrate and add 100 μL of concentrated sulfuric acid to shake well, then take 100 μL of the solution as the solution to be tested, add 400 μL of gold nano-enhancer, add 100 μL of acidifier, shake well and test, according to 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0050] The liquid to be tested is at 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) has a characteristic peak (see figure 1 ), it means that the sample contains ganoderma acid G.

Embodiment 2

[0051] The rapid qualitative analysis of ganoderma acid G in the ganoderma lucidum spore oil of embodiment 2

[0052] Sample: taken from the commercially available Ganoderma lucidum spore oil B.

[0053] a. Weigh 0.4 g of spore oil, add 3.2 mL of anhydrous methanol for oscillation, and place in an ultrasonic extractor to sonicate for 10 minutes.

[0054] b. Shake the extract and filter.

[0055] Qualitative analysis:

[0056] Take 200 μL of filtrate and add 200 μL of concentrated sulfuric acid to shake well, then take 100 μL of the solution as the test solution, add 300 μL of gold nano-enhancer, add 50 μL of acidifier, shake well and test, according to 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0057] The liquid to be tested is at 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) there is no characteristic peak, it means that there is no ganoderma acid G in the sample.

Embodiment 3

[0058] The rapid qualitative analysis of ganoderma acid G in the ganoderma lucidum spore oil of embodiment 3

[0059] Sample: taken from the commercially available ganoderma lucidum spore oil C.

[0060] a. Weigh 0.4 g of spore oil, add 4 mL of anhydrous methanol for oscillation, and place in an ultrasonic extractor for 10 minutes of sonication.

[0061] b. Shake the extract and filter.

[0062] Qualitative analysis:

[0063] Take 200 μL of filtrate and add 40 μL of concentrated sulfuric acid to shake well, then take 100 μL of the solution as the solution to be tested, add 200 μL of gold nano-enhancer, add 100 μL of acidifier, shake well and test, according to 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) to evaluate whether there is a characteristic Raman peak:

[0064] The liquid to be tested is at 555cm -1 (±3cm -1 ), 1176cm -1 (±3cm -1 ) has a characteristic peak (see figure 1 ), it means that the sample contains ganoderma acid G.

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Abstract

The invention relates to a rapid detection technology of ganoderic acid G in ganoderma lucidum spore oil, in particular to a detection method adopting Raman spectrum characteristic peak signals, and the method is suitable for qualitative detection of ganoderic acid G in ganoderma lucidum spore oil. Raman spectrum characteristic peak information is adopted, 555cm<-1> (+ / -3cm<-1 >) and 1176cm<-1> (+ / -3cm<-1>) are selected as characteristic Raman peaks, qualitative analysis is accurate, the whole detection process is completed within 15min, and rapid qualitative, convenient and low-cost detectionis achieved.

Description

technical field [0001] The invention relates to a rapid detection technology of ganoderma acid G in ganoderma lucidum spore oil, in particular to a detection method using Raman spectrum characteristic peak signal intensity, which is suitable for the rapid qualitative detection method of ganoderma acid G in ganoderma lucidum spore oil. Background technique [0002] Ganoderma lucidum spores are the seeds of Ganoderma lucidum, and its inner wall contains light yellow to light yellow-brown oil droplets, while Ganoderma lucidum spore oil is a lipid substance extracted from Ganoderma lucidum spores, which concentrates many active ingredients in Ganoderma lucidum spores, such as triterpenes of Ganoderma lucidum Acids, unsaturated fatty acids and trace elements, etc. Studies have shown that it has anti-tumor, immune regulation, liver protection and other effects, and is widely used in the pharmaceutical industry. [0003] Since there is no unified national standard, most companies u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/28
CPCG01N21/658G01N1/28
Inventor 张兰天张岩郭美娟张斌史国华吴昊
Owner 河北省食品检验研究院
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