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Artificial tear and preparation method thereof

A technology of artificial tears and human umbilical cord, applied in the field of pharmacy, can solve the problems of tear film instability, bactericidal and immune regulation function decline, single component, etc., achieve the effect of reducing retinal damage, improving retinal function, and reducing inflammatory response

Inactive Publication Date: 2020-08-28
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the artificial tear products on the market for dry eye syndrome caused by various reasons are mainly supplemented with liquid and moisturizing. The products themselves have the characteristics of single ingredients and low osmosis. Although these products can temporarily relieve eye dryness Discomfort, but at the same time, it will also dilute the various tear components that are low in the ocular surface of dry eye, such as inorganic salts, lipid transport proteins, lysozyme, lactoferrin, etc. in the tears, making the tears on the surface of the eyeball The membrane becomes more unstable, and the bactericidal and immune regulation functions decline again

Method used

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  • Artificial tear and preparation method thereof

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preparation example Construction

[0052] In an embodiment of the present invention, the method for preparing exosomes from human umbilical cord mesenchymal stem cells comprises the following steps:

[0053] (1) Cultivate a sufficient amount of human umbilical cord mesenchymal stem cells, wash the cells with PBS 48 hours before extracting stem cell exosomes, replace the serum-free medium, and continue to cultivate for 48 hours;

[0054] (2) Collect the serum-free medium, centrifuge at 300×g for 10 min, and take the supernatant;

[0055] (3) Centrifuge at 2000×g for 10 minutes, and take the supernatant;

[0056] (4) Centrifuge at 10000×g for 30 minutes, and take the supernatant;

[0057] (5) Centrifuge at 100000×g for 70min, and take the precipitate;

[0058] (6) The pellet was resuspended in PBS, centrifuged at 100,000×g for 70 min, and the pellet was collected to obtain the human umbilical cord mesenchymal stem cell exosomes.

[0059] In the embodiment of the present invention, the step (1) is specifically:...

Embodiment

[0072] The content of each component of the artificial tears shown in Examples 1-5 is shown in Table 1.

[0073] Table 1

[0074] Example 1 Example 2 Example 3 Example 4 Example 5 Lysozyme / wt% 2.5 4 1 1.5 3.45 Xanthan gum / wt% 0.5 0.01 1 0.75 0.35 Water-soluble hydroxypropyl chitosan / wt% 2 4 0.1 3.5 0.7 Sodium chloride / wt% 0.9 0.8 1 0.86 0.95 Potassium chloride / wt% 0.015 0.02 0.01 0.013 0.018 Calcium chloride / wt% 0.045 0.035 0.055 0.04 0.05 Human umbilical cord mesenchymal stem cell exosomes / wt% 0.0035 0.005 0.0005 0.0009 0.0045 Water for Injection margin margin margin margin margin

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Abstract

The invention discloses an artificial tear and a preparation method thereof, and belongs to the field of pharmacy. The artificial tear comprises 0.0005-0.005 wt% of human umbilical cord mesenchymal stem cell exosome; the artificial tear prepared from exosome derived from human umbilical cord mesenchymal stem cells is used as a drug for local injection of an experimental autoimmune uveitis model ofa rat, and experiments prove that the artificial tear containing exosome can promote corneal ulcer healing, improve recovery rate, reduce corneal scar formation, has an immunosuppressive effect, canrelieve retinal damage caused by various reasons and relieve inflammatory response, so that the retinal function is improved, and a new way is opened up for clinical treatment of ophthalmic diseases.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to an artificial tear and a preparation method thereof. Background technique [0002] In today's information age, computers have become an indispensable tool in people's work and study. However, scientific research has shown that if working in front of the computer for more than three hours a day, 90% of people will have dry eyes, fatigue, difficulty concentrating and other characteristics of dry eye syndrome. [0003] The human eyes, especially the cornea, often rely on the lacrimal gland to supply water. By blinking, the tears become a layer of "tear flakes" and disperse to the cornea to keep the eyes comfortable. The so-called dry eye disease can be said to be a kind of computer vision syndrome, which refers to a symptom in which the eyes show tiny scars due to the decrease of tears or the function of the lacrimal gland. The general symptoms of dry eye disease are dry eyes, burning sens...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K38/47A61K9/08A61K47/36A61P27/04A61P27/02A61P37/02C12N5/0775A61K31/722A61K33/14
CPCA61K9/0048A61K9/08A61K31/722A61K33/14A61K35/28A61K38/47A61K47/36A61P27/02A61P27/04A61P37/02C12N5/0665C12N2509/00C12Y302/01017A61K2300/00
Inventor 王晓冰徐欣
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV