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Method for preparing natural delta-decalactone and delta-dodecalactone by biologically reducing massoia oil

A kind of technology of laurolactone and decanolide, which is applied in the field of biological reduction of massoia oil to prepare natural butyl-decalactone and butyl-decalactone

Active Publication Date: 2020-09-08
XIAMEN OMIC BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the technical scheme disclosed in US5763233, Pseudomonasputida ATCC 33015 is used to ferment and reduce 5-hydroxy-2-decenoic acid δ-lactone for 48 hours to only obtain 10.9g / L butyl-decalactone

Method used

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  • Method for preparing natural delta-decalactone and delta-dodecalactone by biologically reducing massoia oil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The production of embodiment 1 natural butyl decalactone and butyl laurolactone (erlenmeyer shake flask fermentation)

[0033] (1) Strain acquisition

[0034] A strain that can catalyze the reduction of 5-hydroxy-2-decenoic acid δ-lactone to butyl-decalactone was isolated from the marine mud bed. It was identified as Pasteurella by 18Sr DNA and ITS sequencing and named Pasteurella Yeast Saccharomyces pastorianus OMK-70. The strain was deposited in the China Center for Type Culture Collection (China, Wuhan, Wuhan University) on December 27, 2019, and the preservation number is: CCTCC NO: M20191123.

[0035] (2) Activation of strains: draw Saccharomyces pastorianus OMK-70 from a glycerol tube at -80°C and spread it on a solid slant medium, and culture it statically at 30°C for 60 hours to obtain activated strains.

[0036] The formula of the solid slant medium is specifically as follows in terms of mass percentage: 2.5% peptone; 1.5% yeast extract; 2.5% glucose, 1.5% ag...

Embodiment 2

[0042] The production of embodiment 2 natural butyl decalactone and butyl laurolactone (fermentation tank fermentation)

[0043] Steps (1) to (2) are the same as in Example 1;

[0044] (3) Preparation of seed culture solution: Inoculate the obtained activated strains into a 500mL Erlenmeyer shaker flask equipped with 50mL seed medium, and cultivate them at 30°C and 250rpm for 10-12h to obtain a first-grade seed solution; Put all 50mL into a 10L seed tank equipped with 6L seed medium, and cultivate for 6-8h at 30°C, with a stirring speed of 250rpm and an air flow of 1:0.5, to obtain a secondary seed liquid;

[0045] The formula of seed culture medium is with embodiment 1.

[0046] (4) Fermentation and transformation: 16.2L fermentation medium in a 30L fermenter tank, sterilized at 115°C for 30min, and the cultured secondary seed liquid was inserted into a 30L fermenter tank according to the inoculum size of 10% for cultivation, culture conditions: temperature 30 ℃, stirring s...

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Abstract

The invention discloses a method for preparing natural delta-decalactone and delta-dodecalactone by biologically reducing massoia oil. The method comprises the following steps: (1) strain activation;(2) preparation of a seed culture solution; (3) fermentation and converting; (4) extraction; (5) solvent recovery; and (6) rectification. Compared with existing production processes, the method has higher space-time yield, and can obtain higher product concentration. Saccharomyces pastorianus OMK-70 subjected to multiple rounds of mutagenesis is used for fermenting and reducing 5-hydroxy-2-decenoic acid delta-lactone and 5-hydroxy-2-dodecenoic acid delta-lactone in massoia oil so as to generate corresponding delta-decalactone and delta-dodecalactone. The method has higher efficiency, greatly-reduced production cost, and good application prospects.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for preparing natural butyl-decalactone and butyl-laurolactone by biologically reducing masoya oil. Background technique [0002] D-decalactone and D-dodecalactone have a coconut aroma, and they have a creamy aroma at low concentrations. They exist in fruits such as coconuts and raspberries, and are important raw materials for preparing flavors such as milk, cream, coconut, strawberry, and peach. , the market demand is large. [0003] The butyl decanolide and butyl laurolactone currently on the market are mainly chemically synthesized products. With the progress and development of society, human beings' willingness to return to nature is becoming stronger and stronger, which promotes the application of natural flavors in the food industry more and more widely. Compared with chemically synthesized products, natural flavors obtained through b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P17/04C12P17/06C12R1/85
CPCC12N1/16C12P17/04C12P17/06C12R2001/85C12N1/185C12N1/18C12R2001/86
Inventor 曾世超赵晓岚肖春燕林晓慧邢晨光刘刚赵希景
Owner XIAMEN OMIC BIOTECH CO LTD