Method for inducing apoptosis of human acute T lymphocytic leukemia cells by using Shuanghuanglian

A technology of leukemia cells and lymphocytes, which is applied in the field of inducing apoptosis of human acute T lymphocytic leukemia cells, can solve problems such as the effect of Shuanghuanglian that has not been found in research, and achieve the effects of optimizing treatment plans, promoting apoptosis, and inhibiting proliferation

Inactive Publication Date: 2020-09-08
THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Shuanghuanglian has been used clinically for many years, no

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for inducing apoptosis of human acute T lymphocytic leukemia cells by using Shuanghuanglian
  • Method for inducing apoptosis of human acute T lymphocytic leukemia cells by using Shuanghuanglian
  • Method for inducing apoptosis of human acute T lymphocytic leukemia cells by using Shuanghuanglian

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Example one:

[0022] S1. Resuscitate human acute T lymphocyte leukemia cells Jurkat, suspended in 10% fetal bovine serum (Gibco company) RPMI-1640 medium (Hyclone company), and then placed in a saturated humidity incubator at 37°C , CO in the incubator 2 The volume fraction is 5%;

[0023] In the pre-experimental study, it was found that the IC50 of Shuanghuanglian intervention for Jurkat cells for 48 hours was 72.35μg / ml respectively. In this experiment, Shuanghuanglian of 0μg / ml, 100μg / ml, 200μg / ml, 400μg / ml will be selected as the intervention concentration;

[0024] S2. Collect human acute T lymphocyte leukemia cells in logarithmic growth phase and adjust the cell density to 1.5 × 10 5 Pieces / ml to obtain cell suspension;

[0025] S3. Add 90μl of the cell suspension to each well of a 96-well plate, and then add 10μl of Shuanghuanglian at different concentrations to each well; add 10μl of serum-free RPMI 1640 medium to the negative control group, and set a blank zero group ...

Example Embodiment

[0032] Embodiment two:

[0033] S1. Resuscitate human acute T lymphocytic leukemia cells Nalm-6, suspended in 10% fetal bovine serum (Gibco company) RPMI-1640 medium (Hyclone company), and then placed in a saturated humidity incubator at a temperature of 37℃, CO in incubator 2 The volume fraction is 5%;

[0034] In pre-experimental studies, it was found that the IC50 of Shuanghuanglian interfering with Nalm-6 cells for 48 hours was 164.5μg / ml respectively. In this experiment, we plan to choose Shuanghuanglian of 0μg / ml, 100μg / ml, 200μg / ml, 400μg / ml as the intervention concentration;

[0035] S2. Collect human acute T lymphocyte leukemia cells in logarithmic growth phase and adjust the cell density to 1.5 × 10 5 Pieces / ml to obtain cell suspension;

[0036] S3. Add 90μl of the cell suspension to each well of a 96-well plate, and then add 10μl of Shuanghuanglian at different concentrations to each well; add 10μl of serum-free RPMI 1640 medium to the negative control group, and set a bla...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for inducing apoptosis of human acute T lymphocytic leukemia cells by using Shuanghuanglian. The method successfully realizes inhibition of leukemia cell proliferationand promotion of apoptosis of leukemia cells by using Shuanghuanglian, and has important guiding significance for later drug clinical research and further optimization of a treatment scheme of leukemia patients.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for inducing apoptosis of human acute T lymphocytic leukemia cells. Background technique [0002] Leukemia is a malignant clonal disease characterized by abnormal proliferation of bone marrow hematopoietic stem cells, and is the most common malignant tumor in the population. In China, the incidence rate of leukemia is high, and epidemiological surveys have found that the incidence rate is dynamically increasing. In recent years, with the optimization and adjustment of risk grouping and chemotherapy drug dose, CART immunotherapy and bone marrow transplantation, the curative effect of leukemia has been significantly improved. Although the prognosis of most patients has improved significantly, the treatment of leukemia is still a thorny issue, and a large number of patients still suffer from poor treatment and eventually die. At present, most CAR-T therapies are still...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/09
CPCC12N5/0694C12N2500/76
Inventor 刘文君马文哲杨友
Owner THE AFFILIATED HOSPITAL OF SOUTHWEST MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap