Preparation method of chitosan oligosaccharide with specific polymerization degree
A specific degree of polymerization and chitosan oligosaccharide technology, applied in the field of preparation of chitosan oligosaccharides, can solve the problems of wide molecular weight distribution of chitosan oligosaccharides, increase safety hazards, and poor pertinence, etc., to achieve easy control, improve production efficiency, and distribution Narrow Effect
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[0026] A kind of preparation method of the chitosan oligosaccharide of specific polymerization degree, comprises the following steps:
[0027] S1. Purification of chitosan: deacetylation degree ≥ 96%, molecular weight 0.6-1×10 7 Da chitosan is dissolved in 0.8-5.0% lactic acid aqueous solution and dried at 90-110°C to obtain purified chitosan;
[0028] S2, dissolving chitosan: add the chitosan purified in step S1 into the acid solution, place it in an ultrasonic generator, and sonicate until it dissolves under the conditions of ultrasonic power 80-150W, frequency 20-40HZ and temperature 20-40°C. Prepared into 3-5% chitosan solution;
[0029]S3, stage 1 hydrolysis: add non-specific enzymes to the chitosan solution according to the addition amount of 10-30U / g chitosan, adjust the pH to 4.0-5.0, prepare the chitosan reaction solution, and then react the chitosan Place the liquid in an ultrasonic generator, and react for 0.5-1.5h under the conditions of 20-40°C, ultrasonic power...
Embodiment 1
[0036] A kind of preparation method of the chitosan oligosaccharide of specific polymerization degree, comprises the following steps:
[0037] S1. Purification of chitosan: deacetylation degree ≥ 96%, molecular weight 0.6-1×10 7 Da chitosan is dissolved in 0.8-5.0% lactic acid aqueous solution, the mass ratio of chitosan to lactic acid is 5:4-3.5, and dried at 90-110°C to obtain purified chitosan;
[0038] S2, dissolving chitosan: add the chitosan purified in step S1 into 0.8-5.0% lactic acid aqueous solution, the mass ratio of chitosan to lactic acid is 5:4-3.5, place it in an ultrasonic generator, and Under the conditions of 80W, frequency 20HZ, ultrasonic to dissolve, and prepare 5% chitosan solution;
[0039] S3, stage 1 hydrolysis: add compound enzyme to chitosan solution according to the addition amount of 10U / g chitosan, described compound enzyme comprises 5U / g lysozyme and 5U / g pectinase, adjust pH to be 4.0-5.0 , prepared into a chitosan reaction solution, and then ...
Embodiment 2
[0046] The difference between embodiment 2 and embodiment 1 is:
[0047] S3, stage 1 hydrolysis: add pectinase to the chitosan solution according to the addition amount of 20U / g chitosan, adjust the pH to be 4.0-5.0, prepare the chitosan reaction solution, then put the chitosan reaction solution in In an ultrasonic generator, react at 30°C, ultrasonic power 100W, and frequency 30HZ for 1 hour. After the degradation is complete, heat up to 100°C and keep it for 8-13 minutes to inactivate the enzyme. Adjust the pH to 7.5-8.5 for neutralization. Centrifugal filtration to obtain a low molecular weight chitosan solution with a degree of polymerization of 75-110;
[0048] S4, stage 2 hydrolysis: add dextranase to the low molecular weight chitosan solution according to the amount of 45U / g chitosan, adjust the pH to 4.5-6.5, react at 50°C for 1.5h, and heat up after the degradation is completed Keep at 100°C for 8-13 minutes to inactivate the enzyme, adjust the pH to 7.5-8.5 for neut...
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