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Culture medium and culture method for promoting sterile germination of dendrobium officinale seeds

A technology for germination and cultivation of Dendrobium officinale, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low germination rate, slow seed germination speed, acquisition of seedlings, etc., and achieves improvement of germination rate and germination speed. , the effect of high germination rate

Inactive Publication Date: 2020-10-02
安徽皖斛堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Dendrobium candidum mostly adopts the method of tissue culture, using seeds as explants for sterile breeding, the germination rate is low, and the seed germination speed is slow, so it is difficult to obtain a large number of seedlings in a short time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A culture medium for promoting aseptic germination of Dendrobium officinale seeds, comprising the following components: 230 g of potato juice, 1 mg of 6-BA, 0.5 mg of NAA, 0.14 mg of lanthanum nitrate, 15 mg of total saponins of Panax notoginseng, 0.25 g of oligopeptides, and trehalose 0.45g, 28g sucrose, 8g agar, 1 / 2 MS medium to make up to 1000mL.

[0021] Wherein, the oligopeptide is a wheat oligopeptide with a molecular weight of 500-1000Da.

[0022] A kind of aseptic germination culture method of dendrobium officinale seed, comprises the steps:

[0023] S1, get the mature fruit of Dendrobium officinale, after cleaning the epidermis with clear water, soak it in 70% alcohol for 45 seconds, rinse it with sterile water for 3 times, and then treat it with mercuric chloride solution with a concentration of 0.1% for 20 minutes. Rinse with sterile water 10 times, and dry the moisture of the epidermis with sterile filter paper;

[0024] S2. Cut the fruit treated in step S...

Embodiment 2

[0026] A medium for promoting aseptic germination of Dendrobium officinale seeds, comprising the following components: 220 g of potato juice, 0.8 mg of 6-BA, 0.4 mg of NAA, 0.1 mg of lanthanum nitrate, 5 mg of total saponins of Panax notoginseng, 0.1 g of oligopeptides, Trehalose 0.2g, sucrose 25g, agar 7g, 1 / 2 MS medium to make up to 1000mL.

[0027] Wherein, the oligopeptide is a wheat oligopeptide with a molecular weight of 500-1000 Da.

[0028] A kind of aseptic germination culture method of dendrobium officinale seed, comprises the steps:

[0029] S1, get the mature fruit of Dendrobium officinale, after cleaning the epidermis with clear water, soak it in 70% alcohol for 50 seconds, rinse it with sterile water for 3 times, then treat it with 0.05% mercuric chloride solution for 30 minutes, use Rinse with sterile water 10 times, and dry the moisture of the epidermis with sterile filter paper;

[0030] S2. Cut the fruit treated in step S1, sow and inoculate the seeds in th...

Embodiment 3

[0032] A medium for promoting aseptic germination of Dendrobium officinale seeds, comprising the following components: 250g of potato juice, 1.2mg of 6-BA, 0.6mg of NAA, 0.2mg of lanthanum nitrate, 20mg of total saponins of Panax notoginseng, 0.4g of oligopeptides, Trehalose 0.5g, sucrose 30g, agar 10g, 1 / 2 MS medium to make up to 1000mL.

[0033] Wherein, the oligopeptide is a wheat oligopeptide with a molecular weight of 500-1000 Da.

[0034] A kind of aseptic germination culture method of dendrobium officinale seed, comprises the steps:

[0035] S1, get the mature fruit of Dendrobium officinale, after cleaning the epidermis with clear water, soak it in 75% alcohol for 40 seconds, rinse it with sterile water for 3 times, and then treat it with mercuric chloride solution of 0.15% for 15 minutes. Rinse with sterile water 10 times, and dry the moisture of the epidermis with sterile filter paper;

[0036] S2. The fruit treated in step S1 was cut, and the seeds in the fruit wer...

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PUM

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Abstract

The invention discloses a culture medium and a culture method for promoting sterile germination of dendrobium officinale seeds. The culture medium comprises the following components: 220-250 g of potato juice, 0.8-1.2 mg of 6-BA, 0.4-0.6 mg of NAA, 0.1-0.2 mg of lanthanum nitrate, 50-100 mg of total saponins of panax notoginseseng, 0.1-0.4 g of oligopeptide, 0.2-0.5 g of trehalose, 25-30 g of sucrose, 7-10 g of agar and the balance of 1 / 2MS culture medium, wherein the total volume is 1000 mL. According to the culture medium and the culture method disclosed by the invention, by optimizing the formula of the culture medium, the germination rate and germination speed of sterile breeding culture of the dendrobium officinale seeds are effectively increased, and large-scale cultivation of dendrobium officinale is facilitated.

Description

technical field [0001] The invention relates to the technical field of aseptic culture of dendrobium candidum, in particular to a culture medium and a culture method for promoting aseptic germination of dendrobium candidum seeds. Background technique [0002] Dendrobium officinale is a herbaceous plant of the genus Dendrobium orchidaceae. It has a long history of medicinal use. It is rich in polysaccharides, alkaloids, amino acids and other active ingredients. The health care products of Dendrobium candidum are highly praised by the market because of their effects on blood sugar, anti-aging and enhancing immunity. Due to the gradual increase in market demand, the resources of wild Dendrobium officinale are becoming increasingly exhausted, and the price is becoming more and more expensive. Therefore, the artificial cultivation of Dendrobium candidum has become the main source, and the demand for seedlings for the cultivation of Dendrobium candidum is also increasing. At pre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 张建民
Owner 安徽皖斛堂生物科技有限公司