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Angiotensin converting enzyme assay kit as well as preparation method and application thereof

An angiotensin-converting enzyme technology, applied in biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., can solve problems such as poor stability, improve bottle opening stability, and improve anti-bilirubin interference effect of ability

Active Publication Date: 2020-10-16
WUHAN LIFE ORIGIN BIOTECH LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problem of poor stability of existing ACE detection kits, the present invention provides an angiotensin-converting enzyme assay kit, which has better bottle-opening stability and Anti-bilirubin interference ability, while the repeatability, linear range, precision and accuracy of the reagent are not affected

Method used

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  • Angiotensin converting enzyme assay kit as well as preparation method and application thereof
  • Angiotensin converting enzyme assay kit as well as preparation method and application thereof
  • Angiotensin converting enzyme assay kit as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0041] 1. the present invention is provided with 4 comparative examples and 1 embodiment, and the kit composition of each comparative example and embodiment is as shown in table 1:

[0042] The kit composition of table 1 comparative example 1-4 and embodiment 1

[0043]

[0044] 2. Reagent evaluation

[0045] Evaluate the performance index of comparative example 1-4 and embodiment 1 reagent according to the following methods:

[0046] (1) Accuracy: measure third-party quality control products, calculate the deviation between the measured average value and the target value, ≤10% is considered to meet the requirements;

[0047] (2) Linear range: measure samples in the range of 0-200U / L, calculate the correlation coefficient, r≥0.9900, and at the same time in the range of (0, 20] U / L, the absolute deviation does not exceed ±4U / L, in (20, 200 Within the range of ]U / L, the condition that the relative deviation does not exceed ±10% is considered to meet the requirements;

[0048...

Embodiment 1

[0070] In Example 1, stabilizers, potassium ferrocyanide, and Emulgen A90 were added respectively, and the accuracy, bottle-opening stability, and anti-bilirubin interference ability were all significantly improved.

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Abstract

The invention provides an angiotensin converting enzyme assay kit, and belongs to the technical field of biological detection. The kit is composed of a single reagent, wherein the single reagent comprises the following components: 50 to 100 mmol / L of a buffer solution, 200 to 500 mmol / L of an inorganic salt, 5 to 20 mg / L of potassium ferrocyanide, 0.1 to 5.0% of a surfactant, 10 to 100 mg / L of a protective agent, 0.05 to 0.1% of an antiseptic, and 0.5 to 2.0 mmol / L of phenylpropionylaminoglycylglycine; wherein the pH value is 8.20 + / -0.05. Compared with a conventional ACE (Angiotensin Converting Enzyme) detection kit, the kit has better bottle opening stability and bilirubin interference resistance, and meanwhile, the repeatability, the linear range, the precision and the accuracy of the reagent are not influenced. The invention also provides a preparation method and application of the angiotensin converting enzyme assay kit.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and relates to an angiotensin-converting enzyme assay kit, a preparation method and an application. Background technique [0002] Angiotensin-converting enzyme, ACE for short, is a membrane-bound glycoprotein containing zinc ions, with a molecular weight of 150KD. The residue is hydrolyzed to form the pressurizing angiotensin II, which induces vasobronchoconstriction by interacting with angiotensin smooth muscle. [0003] Angiotensin-converting enzyme has the effect of lowering blood pressure, and it can extinguish fire with polypeptide inflammatory substances such as bradykinin substance P. Angiotensin-converting enzyme (ACE) can also directly act on the adrenal cortex to promote the secretion of aldosterone. Therefore, vascular Tensin-converting enzyme is an important regulator of the renin-angiotensin-aldosterone system and the bradykinin system, affecting various physiological f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/37
CPCC12Q1/37G01N2333/948
Inventor 凡速朋龚婷张超舒芹赵愿安
Owner WUHAN LIFE ORIGIN BIOTECH LTD
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