Penicillin bottle preservation method for strains
A technology for preservation of vials and strains, which is applied in the field of microbial engineering, and can solve problems such as increasing the storage capacity of strains in the laboratory, insufficient strain preservation capacity, and reducing storage space for strain preservation
Inactive Publication Date: 2020-10-23
HEBEI CHEM & PHARMA COLLEGE
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- Description
- Claims
- Application Information
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Problems solved by technology
[0004] In view of the above analysis, the present invention aims to provide a vial preservation method for strains, which reduces the storage space used for strain preservation, increases the storage capacity of strains in the laboratory, and solves the problem of insufficient strain preservation capacity
Method used
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[0047] Current status investigation: A laboratory has only one 2-8°C freezer for the preservation of working strains, and needs to preserve standard strains from both ATCC and CMCC sources. Commonly used standard strains (4 strains) need to prepare 30 working strains for each strain, and generally 2-10 working strains are prepared for each of the uncommonly used strains (4 strains), resulting in a shortage of storage space for the strains.
[0048] The material of the vial is the same as that of the test tube, and its volume is smaller than that of the test tube. It can be placed upright independently, can be sterilized, and can be recycled. The volume of the vial is 7ml to 25ml, and the volume of the test tube currently used for strain preservation is 50ml or 110ml.
[0049] The dimensions of commonly used vials are shown in Table 2 below:
[0050]
[0051]1. Preparation of flat culture medium in vials: Dispense the solid medium prepared according to the instructions and ...
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The invention provides a penicillin bottle preservation method for strains, and belongs to the technical field of microbial engineering. The method comprises the following steps: (1) preparation of apenicillin bottle plane culture medium: subpackaging a prepared solid culture medium of which the pH value is adjusted into a penicillin bottle while the culture medium is hot, covering the bottle with a rubber plug, wrapping the bottle with head wrapping cloth and performing sterilizing; (2) inoculation and culture: inoculating strains into the culture medium in the penicillin bottle, sealing thebottle and performing culturing; (3) culture result confirming: observing the state of a culture after the culture is finished, confirming colony characteristics, observing thallus morphology throughmicroscopic examination, and confirming thallus characteristics; and (4) strain preservation: putting the strains into an environment of 2-8 DEG C for preservation. The penicillin bottle replaces a test tube to serve as a strain preservation container, so that the storage space for strain preservation is reduced, the strain preservation capacity of a laboratory is increased, and the problem of insufficient strain preservation capacity is solved.
Description
technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a method for preserving strains. Background technique [0002] Preservation of strains is essential in microbial research and practical applications, especially in the field of aseptic control, which is the key point in the production control of sterile preparations. Preserving representative or indicative strains is essential for improving the level of verification and improving The level of aseptic technology is of great significance. For example: microorganisms detected in sterilized or disinfected items can be preserved and used as test bacteria for item sterilization or disinfection verification. [0003] With the continuous maturity of microbial identification technology, many companies have the ability to identify the detected microorganisms and store representative or indicative strains. However, due to limited storage space and large labor costs for conven...
Claims
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Login to View More IPC IPC(8): C12N1/04C12R1/19C12R1/125C12R1/145C12R1/445C12R1/385C12R1/44C12R1/725C12R1/685
CPCC12N1/04
Inventor 张静
Owner HEBEI CHEM & PHARMA COLLEGE