Polypeptide monolayer film with primary amino exposure of 5% and preparation method and application thereof
A single-layer film and primary amino technology, applied in the field of natural polymers, can solve problems such as limiting the application of polypeptide molecules, and achieve the effects of improving attachment and proliferation, promoting cell activity, and good compatibility
Active Publication Date: 2020-10-30
QILU UNIV OF TECH
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Problems solved by technology
[0005] Due to the complexity of the structure of natural biological macromolecules, there are few reports on the surface properties of polypeptide monolayer membranes, which limits the application of polypeptide molecules.
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Embodiment 1
[0052] A method for preparing a polypeptide monolayer film, comprising the following steps:
[0053] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.
[0054] (2) Add surfactant G-SHxaS to the above collagen polypeptide solution to obtain collagen polypeptide-G-SHxaS mixed solution, the concentration of G-SHxaS in the mixed solution is 6.83mmol / L (at 50°C, G-SHxaS is mixed The mass fraction in the solution is 6%); it is stable in a water bath for 6h and is ready for use.
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Abstract
The invention provides a polypeptide monolayer film with the primary amino exposure amount of 5% and a preparation method and application of the polypeptide monolayer film. The polypeptide is composedof polypeptide molecules with the molecular weight of (1.48 + / - 0.2)*10 <5> g / mol, the thickness of a monolayer film is 15.2 + / - 0.1 nm, the exposure amount of primary amino groups on the surface ofthe film is 5 + / - 0.2%, the Zeta potential of the polypeptide monolayer film is -4.97 + / - 0.1 mV, and the contact angle of the film is 50 + / - 1 degrees. The specific Zeta potential, the positive charge, the hydrophilicity, the cytocompatibility, the surface wettability and the like of the polypeptide monolayer membrane under the exposure amount can promote the cell activity, have good compatibility with human bone tissue and are beneficial to rapid healing of a sick body. Due to the weak hydrophilic property, attachment and proliferation of cells at the planting position can be improved, and no harm is caused to body tissue after the implant is implanted into the human body.
Description
technical field [0001] The invention belongs to the field of natural polymers, and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with a primary amino group exposure of 5%, and its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Bio-immobilized coatings are often used in the field of biomimetic scaffolds to solve the problem of carrying biomolecules, drug molecules, synthetic polymers or small organic molecules such as enzymes, lactose, and polydopamine. The loading capacity is ea...
Claims
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IPC IPC(8): A61L27/06A61L27/34A61L27/50
CPCA61L27/06A61L27/34A61L27/50A61L2430/02A61L2420/08A61L2420/02C08L89/00C08L79/02
Inventor 许静马慧君李天铎班青张震
Owner QILU UNIV OF TECH