Low-pressure gene delivery device
A gene and low-voltage technology, applied in the field of low-voltage gene delivery devices, can solve problems such as increased cost and difficulty, poor quality of gene guns, and disturbed flow, so as to reduce production difficulty and production cost, improve yield and reliability, and facilitate The effect of processing and manufacturing
Pending Publication Date: 2020-10-30
庄丰如
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Problems solved by technology
However, the above-mentioned propulsion device is bulky, and the high-pressure gas used to propel the metal particles is more likely to cause cell damage
[0004] In addition, the structure of the currently used gene guns is complex, which not only increases the cost and difficulty of production, but also may result in poor quality of the gene guns produced, resulting in high-pressure gas that easily forms gas turbulence, thereby affecting the effect of gene transfer
Method used
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[0034] see figure 1 and figure 2 , are respectively a schematic cross-sectional view and a side view of an embodiment of the low-voltage gene delivery device of the present invention. As shown in the figure, the low-pressure gene delivery device 10 mainly includes a tube body 11 and a partition unit 13, wherein the tube body 11 includes an accommodating space 12, and the partition unit 13 is located in the accommodating space 12 of the tube body 11, and The accommodating space 12 is divided into two connected spaces, such as a pressure accumulating space 121 and an injection space 123 .
[0035] The pipe body 11 includes an inner pipe wall 112, a connection port 111 and a discharge port 113, and an accommodation space 12 is formed between the connection port 111, the discharge port 113 and the inner pipe wall 112, wherein the gas is sprayed from the connection port 111. delivery in the direction of exit 113. In an embodiment of the present invention, the accommodating spac...
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The invention provides a low-pressure gene delivery device which mainly comprises a tube body and a separation unit. The separation unit is arranged in an accommodating space of the tube body and divides the accommodating space into a pressure storage space and a jet space; the separation unit can form a contraction part at one end of the pressure accumulation space and form an expansion part at one end of the injection space. The gas is transmitted from the pressure accumulation space to the jet space, and the speed of the gas is continuously increased when the gas passes through the contraction part so that the DNA solution can be accelerated through the high-speed gas, and the purpose of gene transfection can be achieved on the premise of not using metal particles. The low-pressure genedelivery device is simple in structure, easy to process and manufacture, and beneficial to reducing the production cost of products and improving the yield of the products.
Description
technical field [0001] The invention relates to a low-pressure gene delivery device, which accelerates a solution through gas, so that the solution can penetrate the skin or cell membrane. Background technique [0002] The gene gun is a gene transfer device, which mainly attaches DNA to the surface of metal particles, such as tungsten or gold powder, and accelerates the metal particles through high-pressure gas, so that the metal particles with DNA are ejected and can penetrate skin tissue, The cell wall or membrane, which in turn transports the DNA into the cell. At present, gene guns are widely used in the fields of genetic transformation of animals and plants, DNA vaccines, gene therapy and medical beauty. [0003] The gene gun can use gunpowder explosion, high voltage or high pressure gas as a power source to accelerate metal particles. However, the above-mentioned propelling device is bulky, and the high-pressure gas used to propel the metal particles is more likely t...
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IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 庄咏岚
Owner 庄丰如