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Application of ddrgk1 as a molecular marker to predict the prognosis of patients with gastric cancer treated with platinum-based chemotherapy drugs

A chemotherapeutic drug, a technology for gastric cancer, applied in the field of biomedicine, can solve the problems that are not disclosed in the literature, and achieve the effect of good effect, good prognosis and low drug resistance

Active Publication Date: 2021-05-25
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no literature revealing the relationship between DDRGK1 and tumor drug resistance and the prognosis of cancer patients using chemotherapy drugs

Method used

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  • Application of ddrgk1 as a molecular marker to predict the prognosis of patients with gastric cancer treated with platinum-based chemotherapy drugs
  • Application of ddrgk1 as a molecular marker to predict the prognosis of patients with gastric cancer treated with platinum-based chemotherapy drugs
  • Application of ddrgk1 as a molecular marker to predict the prognosis of patients with gastric cancer treated with platinum-based chemotherapy drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Correlation analysis between the protein expression of DDRGK1 in gastric cancer cells and the drug sensitivity of gastric cancer cells to platinum-based chemotherapy drugs

[0059] Detection objects: gastric cancer cells MGC803, BGC823, SGC7901 and AGS

[0060] experimental method:

[0061] 1. CCK-8 cell proliferation experiment

[0062] Gastric cancer cells were inoculated on a 96-well plate, and the amount of inoculated cells in each well was 1×10 3 cells (cell proliferation experiment) or 8×10 3 cells (drug sensitivity experiment), set 4–5 duplicate wells, after the cells adhered to the wall (12h), start to collect cells at different time points, and detect after adding different concentrations of cisplatin and its control treatment (DMSO) cells Detection of cell proliferation (drug sensitivity test). When testing, first prepare the CCK-8 premix, then take out the 96-well plate, discard the original medium in the well, add 100mL of CCK-8 premix to each well, and ...

Embodiment 2

[0079] Detection of DDRGK1 protein expression in 124 gastric cancer tissue samples collected by immunohistochemistry

[0080] Patient data collection: Cases were screened from the electronic case system of the Second Affiliated Hospital of Soochow University. The inclusion criteria of the cases included: gastric cancer diagnosed by electron microscope pathology; surgical resection of gastric cancer between January 2011 and December 2015 ; Only use platinum-based chemotherapy after surgery; use platinum-based chemotherapy ≥ 3 cycles. According to the 7th edition of AJCC, gastric cancer patients who met the inclusion criteria were classified into TNM staging, and electronic medical records were reviewed to obtain the disease-free survival (DFS) of patients.

[0081] SP method (streptavidin-peroxidase) method to detect the protein expression of DDRGK1 in gastric cancer tissue: formalin-fixed, paraffin-embedded gastric cancer tissue specimens were obtained from patients who met th...

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Abstract

For the first time, the present invention studies the relationship between the expression of DDRGK1 in gastric cancer tissue and the sensitivity to platinum-based chemotherapy drugs, and finds that the protein expression of DDRGK1 is closely related to the drug resistance and prognosis indicators of gastric cancer patients treated with platinum-based chemotherapy drugs. DDRGK1 Patients with high expression have good treatment effect with platinum-based chemotherapy drugs, low drug resistance, and good prognosis, and are suitable for chemotherapy with platinum-based chemotherapy drugs; while patients with low-expression gastric cancer have poor prognosis and treatment effect with platinum-based chemotherapy drugs, and appear The possibility of drug resistance is high, which indicates poor prognosis, and it is not suitable to use platinum chemotherapy drugs for chemotherapy; at the same time, it can formulate individualized treatment plans for different patients, improve the treatment effect, and improve the quality of life of patients; the invention is useful for clinical applications and There is great value in drug development.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the application of DDRGK1 as a molecular marker for predicting the prognosis of gastric cancer patients using platinum-based chemotherapy drugs; Use in kits and compositions. Background technique [0002] Gastric cancer is one of the most common malignant tumors in the world, and its case fatality rate ranks third among tumors. Gastric cancer is also the third largest tumor in my country. At present, surgical resection is the only possible cure for gastric cancer, but it is only effective for early gastric cancer, and most patients are diagnosed at the middle and advanced stages, losing the chance of surgical cure. As one of the first-line chemotherapy drugs for patients with advanced gastric cancer, platinum-based drugs are widely used in clinical practice. However, the 5-year survival rate of these patients is still less than 30%, mainly due to tumor drug resistance and adv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57446G01N33/57496G01N33/68G01N2333/47
Inventor 胡展红王晓卉许国强
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV