Extraction and purification method of tea bran polysaccharide and scalp care composition containing tea bran polysaccharide
A purification method and tea bran technology, which are applied in hair care, skin care preparations, cosmetics and other directions, can solve problems such as no reports of efficacy, and achieve the effects of inhibiting growth, avoiding irritation, and high protein removal rate
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Embodiment 1
[0030] Extraction method of tea gluten polysaccharide:
[0031] (1) Take the tea bran powder of defatted camellia oleifera, add 85% ethanol, reflux for 2 hours under the condition of solid-liquid ratio 1:7, temperature 60°C, and dry the filter residue after suction filtration to obtain the tea bran powder without thea saponin;
[0032] (2) Mix the tea bran powder and distilled water, and extract for 3 hours under the condition of material-to-liquid ratio of 1:7 and temperature of 70°C. After the extract is centrifuged, the supernatant is taken, and the volume of the supernatant is evaporated under reduced pressure. Concentrate to 20% of the original volume;
[0033] (3) Adjust the pH of the concentrated extract obtained in (2) to 4.5 with dilute hydrochloric acid at room temperature, and centrifuge to remove the precipitate after standing for 12 hours;
[0034] (4) prepare ammonium sulfate / polyethylene glycol (PEG) 2000 two-phase extraction system, wherein ammonium sulfate ma...
Embodiment 2
[0043] After concentrating and desalting the tea gluten polysaccharide obtained in Example 1, adopt DEAE cellulose column chromatography for molecular weight fractional purification: DEAE-52 cellulose pretreatment: take DEAE-52 cellulose, add distilled water and place at 4°C Swell for 48 hours, then filter with suction; soak in 0.5mol / L NaOH solution for 2 hours, wash with distilled water until pH = 7.0, and filter with suction; then soak in 0.5mol / L HCl solution for 2 hours, wash with distilled water until pH = 7.0, and filter with suction ;Finally soak in 0.5mol / L NaOH solution for 2h, wash with distilled water to pH=7.0, soak it in distilled water, and remove air bubbles by ultrasonic; - Slowly pour the cellulose suspension into the column, keep the outlet flow rate stable, let it settle naturally, add an appropriate amount of quartz sand, and use distilled water to balance the chromatography column; sample loading and elution: take 200 mg of the above-mentioned lower phase ...
Embodiment 3
[0048] Preparation of scalp care solution: in terms of mass percentage (g: mL), including 2% tea bran polysaccharide (tea bran polysaccharide prepared in Example 1, tea bran polysaccharide component A, tea bran polysaccharide component B or tea bran polysaccharide One of the polysaccharide component C), 0.8% Sophora flavescens extract, 0.5% ginsenoside, 2% glycerin, 3% butanediol, 1% menthol, 1% hydrogenated lecithin, 1% 1,2-pentane Glycol, 0.1% zinc aspartate, 0.05% copper PCA, 0.05% EDTA-2Na, 0.1% citric acid, 0.2% arginine, 0.2% sarcosine, 0.3% sodium benzoate, 5% ethanol, balance for water.
[0049] The scalp care solutions made by the above four groups are successively recorded as scalp care solution 1 (adding the tea bran polysaccharide group obtained in Example 1), scalp care solution 2 (adding tea bran polysaccharide component A), scalp care solution 3 ( Add tea bran polysaccharide component B), scalp care solution 4 (add tea bran polysaccharide component C). Volunte...
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