HPV sample collection and preservation card and preparation method thereof
A technology for preserving cards and soaking liquids, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as affecting the accuracy and sensitivity of PCR results, increasing experimental operation steps and sample exposure, and being expensive. The effect of effectively killing bacteria, simple manufacturing process and easy operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0032] Embodiment 1: the preparation of HPV storage card
[0033] 1) Prepare HPV soaking solution: dissolve 19.38 g Tris, 212.688 g guanidine isothiocyanate, and 0.2 g bromophenol blue in 900 ml nuclease-free water, adjust the pH to 11.0 with NaOH, add nuclease-free water to make up to 1L;
[0034] 2) Preservation medium treatment: immerse 903 filter paper (580 mm X 580 mm) in a certain volume of HPV soaking solution (according to 50 μl solution / cm 2 The ratio of the medium), placed in a closed container to prevent external DNA contamination, 100 rpm / min shaking for 5-10 minutes, until the solution is completely absorbed;
[0035] 3) Drying: Carefully take out the filter paper that has absorbed the HPV immersion solution, bake at 80°C for 1 hour, fully dry and take out. Note: When drying, the filter papers are stacked, and one is pressed against the other to ensure that the two overlap;
[0036] 4) Cutting and bagging: use the indentation device to press out multiple 45 mm X...
Embodiment 2
[0037] Embodiment 2: HPV storage card bacteriostasis performance test
[0038] Species: Escherichia coli (gram-negative bacteria), Enterococcus faecium (gram-positive bacteria), Candida albicans (fungus)
[0039] Control substance: FTA card (GE Healthcare)
[0040] Negative control substance: 903 filter paper (3M company)
[0041] Take 200 μl of overnight cultured Escherichia coli and Enterococcus faecium, spread them on LB solid medium respectively, spread 200 μl of overnight cultured Candida albicans on SDA medium, and cut the HPV storage card and reference substance FTA card to 1cm 2 Put it on the LB medium and SDA medium coated with bacteria, place it in an incubator at 37°C, and observe the growth of Escherichia coli and Enterococcus faecium after 12 hours of cultivation, and observe the growth of Candida albicans after 2-3 days of cultivation of growth.
[0042] The result is as figure 1 As shown, the 903 filter paper of the negative control substance without treatme...
Embodiment 3
[0043] Embodiment 3: sample collection, preservation and obtain the DNA of purification from HPV sample card
[0044] (1) HPV sample collection: Use a disposable cervical sampling brush to collect cervical or vaginal secretions, and then apply the collection brush head with HPV samples to the sample storage area on the HPV card repeatedly for 3-4 times. It will change from purple to white or beige within a few minutes. Place the sample card at room temperature until it is completely dry.
[0045] (2) Information filling and card packaging: After filling in the sample information, put the card with the HPV sample in the sample collection bag, seal it and store it at room temperature.
[0046] (3) Use of DNA on the HPV card: Use a hole punch, scissors or a disposable blade to take a small piece of the card containing the HPV sample area (yellow area), put it in a 0.2 ml centrifuge tube, add 100 μL of nuclease-free water, and Shake on a horizontal shaker for 1 minute, discard th...
PUM
| Property | Measurement | Unit |
|---|---|---|
| diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More