Botanical ecdysone used for preventing muscle strength loss during fixation
An ecdysone, plant-based technology, applied in the field of plant-derived ecdysone and its derivatives for preventing muscle loss during fixation, can solve problems such as difficulty in large-scale introduction of procedures and intractable muscle areas to be stimulated.
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[0088] The method for synthesizing formula (II) compound
[0089] The compounds of formula (II) cited in the remainder of the specification are as follows:
[0090]
[0091] The compound of formula (II) is obtained by semi-synthesis from 20-hydroxyecdysone and subsequent purification to pharmaceutical grade.
[0092] Processes for the preparation of compounds of formula (II) by semi-synthesis include in particular:
[0093] - a step of oxidative cleavage of the side chain of 20-hydroxyecdysone between carbons C20 and C22 in order to obtain poststerone,
[0094] - the step of introducing a bromine atom at position C21, and
[0095] - a step of reacting a bromine derivative with ethanethiol.
[0096] Biological activity of compounds of formula (II)
[0097] The immobilization model of the hind paw of genetic background C57BL / 6J mice was implemented by tube (Lang et al., 2012).
[0098] Female C57BL / 6J mice aged 13 weeks were used. Ten mice were sacrificed at J0 and ...
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