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Method for producing selenium-enriched yeast using isomaltulose mother liquor and chlorella waste liquor

A technology of isomaltulose and selenium-enriched yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, can solve the problems of high cost and low purity, and achieve cost-saving effects

Active Publication Date: 2022-05-10
QINGDAO KEHAI BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The object of the present invention is to provide a method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquor, which utilizes isomaltulose mother liquor and chlorella waste liquor as raw materials to turn waste into wealth and realize The concept of industrial circular economy development is to process these two components for the selenium-enriched culture of Candida utilis to realize the conversion of old and new kinetic energy, so as to solve the problems of high cost and low purity in the current production process of selenium-enriched yeast. Effective utilization of maltulose mother liquor and chlorella waste liquor to create economic benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquor specifically comprises the following steps:

[0033] (1) Take the mother liquor after crystallization of isomaltulose, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0034] (2) Take 18 kg of cornmeal, add 100 L of water, add amylase and glucoamylase and incubate at 60°C for 5 hours, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0035] (3) Mix the solutions obtained in steps (1) and (2) with the chlorella waste liquid in a ratio of 1:1:2, and sterilize at 115°C for 20 minutes for later use.

[0036] (4) Seed preparation: under aseptic conditions, a ring of Candida utilis was picked from the preserved slant and inoculated on the sterilized YPD basal medium, cultured on a shaker at 30°C and 180r / min for 16h;

[0037] (5) Nutrient composition transition: Take ...

Embodiment 2

[0043] The method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquor specifically comprises the following steps:

[0044] (1) Take the mother liquor after crystallization of isomaltulose, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0045] (2) Take 18 kg of cornmeal, add 100 L of water, add amylase and glucoamylase and incubate at 55°C for 5 hours, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0046] (3) Mix the solutions obtained in steps (1) and (2) with the chlorella waste liquid in a ratio of 1:1:2, and sterilize at 115°C for 20 minutes for later use.

[0047] (4) Seed preparation: under aseptic conditions, a ring of Candida utilis was picked from the preserved slant and inoculated on the sterilized YPD basal medium, cultured on a shaker at 30°C and 180r / min for 16h;

[0048] (5) Nutrient component transition: Take th...

Embodiment 3

[0054] The method for producing selenium-enriched yeast by using isomaltulose mother liquor and chlorella waste liquor specifically comprises the following steps:

[0055] (1) Take the mother liquor after crystallization of isomaltulose, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0056] (2) Take 18 kg of cornmeal, add 100 L of water, add amylase and glucoamylase and incubate at 55°C for 5 hours, measure its sugar content with an Abbess refractometer, and dilute to a sugar content of 10°Be'.

[0057] (3) Mix the solutions obtained in steps (1) and (2) with the chlorella waste liquid in a ratio of 2:1:3, and sterilize at 115°C for 20 minutes for later use.

[0058] (4) Seed preparation: under aseptic conditions, a ring of Candida utilis was picked from the preservation slant and inoculated on the sterilized YPD basal medium, cultured on a shaker at 30°C and 180r / min for 16h;

[0059] (5) Nutrient component transition: Take...

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Abstract

The invention discloses a method for producing selenium-enriched yeast by utilizing isomaltulose mother liquor and chlorella waste liquor, and relates to the technical field of yeast preparation. The first step is to pre-treat isomaltulose mother liquor and corn flour, and then mix it with chlorella waste liquor in a certain proportion for the cultivation of Candida utilis. According to the physiological and metabolic characteristics of the strain, selenium nitrite is added twice Selenium-enriched culture of yeast. The present invention realizes for the first time that isomaltulose mother liquor and chlorella waste liquid are used in the production of selenium-enriched yeast. The present invention utilizes isomaltulose mother liquor and chlorella waste liquid as raw materials, turns waste into wealth, and realizes industrial The idea of ​​circular economy development is to process these two components for the selenium-enriched culture of Candida utilis to realize the conversion of old and new kinetic energy, so as to solve the problems of high cost and low purity in the current production process of selenium-enriched yeast. The ketose mother liquor and the chlorella waste liquor are effectively utilized to achieve the purpose of creating economic benefits.

Description

technical field [0001] The invention relates to the technical field of yeast preparation, in particular to a preparation method of selenium-enriched yeast. Background technique [0002] As an essential nutrient element for the human body, selenium plays an important physiological function in the life activities and metabolism of the human body. The anti-oxidation effect of selenium is outstanding. The active center components of several antioxidant enzymes in the human body are composed of selenium, such as the peroxidase glutathione peroxidase that exists in humans and animals. Active center (selenocysteine). Selenium can improve the immunity of the body. Selenium exists in almost all immune cells, such as vitamin E and coenzyme Q, all of which require the participation of selenium to activate immune cells and enhance the immune function of the body. Selenium also has the functions of participating in the basic metabolism of the body, antagonizing heavy metal detoxificati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12N1/16C12R1/72
CPCC12N1/38C12N1/16
Inventor 李悦明徐建春梁荣荣韩萍徐炳政
Owner QINGDAO KEHAI BIOLOGICAL