Unlock instant, AI-driven research and patent intelligence for your innovation.

Liquid crystal-based detection probe and preparation method and application thereof

A technology for detecting probes and liquid crystals, which is applied in the field of biochemical sensors, can solve problems such as complicated detection, and achieve the effects of good specificity, convenient and fast detection, and simple detection methods

Pending Publication Date: 2020-11-24
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
View PDF8 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Research on the application of liquid crystal materials to ion detection already exists, but the realization of ion detection often requires the use of more professional equipment, and the detection is more cumbersome.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liquid crystal-based detection probe and preparation method and application thereof
  • Liquid crystal-based detection probe and preparation method and application thereof
  • Liquid crystal-based detection probe and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] This embodiment provides a liquid crystal-based detection probe, the preparation method of which is as follows: figure 1 As shown, it specifically includes the following steps:

[0069] (1) Add 30% H 2 o 2 and H 2 SO 4 Mix 1:1 to obtain piranha solution, wash the glass substrate at 110°C for 30 minutes, wash with deionized water and air dry;

[0070] (2) Soak the glass substrate with an ethanol solution containing 0.1% APTES and DMOAP with a mass concentration of 0.1%, coat the surface of the glass substrate with a GA solution with a mass concentration of 0.5%, and air-dry it with nitrogen;

[0071] (3) Soak the upper surface of the glass substrate in a buffer solution containing nucleotide chain A and nucleotide chain B at 37°C, the concentration of nucleotide chain A is 200 μM, and the concentration of nucleotide chain B is 200 μM;

[0072] After soaking for 1 hour, wash the surface with deionized water;

[0073] Wherein, the sequence (SEQ ID NO.1) of nucleotide...

Embodiment 2

[0080] This embodiment provides a detection probe based on liquid crystal. The difference between its preparation method and that of Example 1 is that in the buffer solution of nucleotide chain A and nucleotide chain B, the concentration of nucleotide chain A is 100 μM, and the The concentration of nucleotide chain B is 100 μM; other steps are the same as in Example 1.

Embodiment 3

[0082] This embodiment provides a detection probe based on liquid crystal. The difference between its preparation method and that of Example 1 is that in the buffer solution of nucleotide chain A and nucleotide chain B, the concentration of nucleotide chain A is 500 μM, and the The concentration of nucleotide chain B is 500 μM; other steps are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a liquid crystal-based detection probe and a preparation method and application thereof. The detection probe comprises a substrate layer, a nucleotide chain A connected to the substrate layer, a nucleotide chain B connected with the nucleotide chain A through a base complementary pairing effect, and nematic phase liquid crystals vertically arranged on the substrate layer. Aliquid crystal layer is vertically anchored to the glass substrate through dimethyl octadecyl [3-(trimethoxysilyl) propyl] ammonium chloride, 3-aminopropyltriethoxysilane, glutaraldehyde and dodecyl trimethyl ammonium bromide are connected together through interaction of nucleotide chains, and liquid crystals are assisted in vertical arrangement. When the lead ions are detected, liquid crystals are arranged disorderly due to the fact that the lead ions have a shearing effect on nucleotide chains, scattering of different degrees in all directions occurs after light is incident, the brightness under an orthogonal polarizing microscope changes, and then the concentration of the lead ions is detected through the brightness gray value intensity.

Description

technical field [0001] The invention relates to the field of biochemical sensors, in particular to a liquid crystal-based detection probe and its preparation method and application. Background technique [0002] With the progress of the times and society, people pay more and more attention to the problems of personal health and environmental pollution, and the heavy metal ions have great harm to the human body and the environment, which has attracted people's attention and research. For example, lead ions, reaching a certain level in the human body will lead to brain memory loss, emotional instability, cardiovascular disease, and muscle paralysis in young children. Lead ion is a non-degradable substance that exists in our living environment. The maximum lead ion content in general human drinking water is 15μg / L or 72nM. [0003] The traditional lead ion detection methods mainly include electrochemical method, colorimetric method, anodic stripping voltammetry and so on. Alt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/84C12Q1/6816G01N21/41
CPCG01N21/84G01N21/41C12Q1/6816
Inventor 罗丹杜晓雪牛小方
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA