Method for detecting antibiotics by fluorescence sensor based on aptamer

Abstract

The invention discloses a method for detecting antibiotics by a fluorescence sensor based on an aptamer. The method comprises the following steps of: selecting a DNA single strand capable of being specifically combined with antibiotics as an aptamer, designing multiple complementary strands having different pairing strengths with the aptamer, and performing detection by combining the characteristics of a fluorescent dye and double-stranded DNA. When the antibiotics are added, complementary strands of the antibiotics and the aptamer compete to be combined with the aptamer; the complementary strands are released, and then completely sheared by exonuclease I (Exonuclease I) added subsequently; and then, report detection on the DNA concentration is carried out by utilizing a double-stranded specific fluorescent dye, so that the relationship between the antibiotic concentration and the fluorescence intensity is obtained, and quantitative analysis is carried out. The method in the inventionhas the advantages of being simple and convenient to operate, small in required sample amount, high in sensitivity and good in practical value, and is expected to be applied to antibiotic detection inmedical treatment, environment and livestock breeding.

Description

technical field [0001] The invention relates to a biosensor detection method, in particular to a nucleic acid aptamer-based fluorescent sensor detection method for antibiotics. Background technique [0002] Antibiotics are widely produced and used. According to statistics, about 6,000 tons of antibiotics are used as feed additives in China alone every year. 75% of antibiotics are not absorbed by animals and are excreted with animal feces, resulting in livestock and poultry farming wastewater becoming antibiotics in natural water bodies. major source of pollution. Antibiotic residues have very serious hazards to human health, including carcinogenicity, teratogenicity, mutagenicity, and long-term exposure of the human body to such drugs, which usually do not cause acute poisoning, but mainly cause chronic poisoning. Antibiotic pollution has been regarded as a new type of important water pollutant and has become a frontier topic of international research. [0003] Sulfonamide...

AI Technical Summary

Problems solved by technology

Long-term use can easily damage kidney function and nervous system, and cause sulfa drug poisoning

[0004] The existing antibiotic analysis and detection methods are cumbersome and rely on large-scale instruments such as HPLC and LC-MS. The time required for detection and the sensitivity need to be further improved. The antibiotic content in the required sample can only be detected when it reaches a certain level, so further exploration is needed for better performance. Analytical method

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