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Photoacoustic/fluorescent bimodal imaging method and device suitable for abdominal visceral organs ofanimal

A dual-mode imaging and internal organ technology, which is applied in the fields of biological science and medical devices and appliances, can solve the problems of visceral organs in the abdominal cavity and other problems, and achieve good biocompatibility, good application prospects, and excellent light transmittance effects

Active Publication Date: 2020-12-04
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a method and device suitable for photoacoustic / fluorescence dual-modal imaging of abdominal internal organs of animals. The abdominal window material sheet that meets the requirements of photoacoustic microscopic imaging and fluorescent imaging is selected at the same time. The idea of ​​assembling into a "drawer type" can further solve the problem of visceral visceral vibration caused by animal breathing and heartbeat jitter, and lay a foundation for the imaging research of abdominal visceral organs

Method used

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  • Photoacoustic/fluorescent bimodal imaging method and device suitable for abdominal visceral organs ofanimal
  • Photoacoustic/fluorescent bimodal imaging method and device suitable for abdominal visceral organs ofanimal
  • Photoacoustic/fluorescent bimodal imaging method and device suitable for abdominal visceral organs ofanimal

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Embodiment 1

[0044] This embodiment provides a photoacoustic / fluorescence dual-mode imaging device suitable for the abdominal organs of animals, such as image 3 As shown, it includes a belly window ring 1, the top surface of the belly window ring 1 is bonded with a circular transparent cover sheet 2, the cover sheet 2 completely covers the window of the belly window ring 1, and the cover sheet 2 and the belly window ring 1 The inner wall is interference fit and bonded by biological tissue glue;

[0045] The bottom surface of the ventral window ring 1 is bonded with a semicircular transparent support piece 3, the arc edge of the support piece 3 is bonded to the inner wall of the ventral window ring 1 through biological tissue glue, and the support piece 3 covers the ventral window ring 1 50% of the window area;

[0046]The supporting sheet 3 and the cover sheet 2 are made of acrylic material with a thickness of 1.0mm.

Embodiment 2

[0048] This embodiment provides a photoacoustic / fluorescence dual-mode imaging device suitable for the abdominal organs of animals, as shown in Figures 1 and 2, including a ventral window ring 1, and the top surface of the ventral window ring 1 is glued with biological tissue Connected with a circular transparent cover 2, the cover 2 is bonded to the top surface of the ventral ring 1, and the cover 2 completely covers the window of the ventral ring 1;

[0049] The bottom surface of the abdominal window ring 1 is bonded with a semicircular transparent support piece 3, and the support piece 3 is bonded to the top surface of the abdominal window ring 1 by biological tissue glue, and the support piece 3 covers the window area of ​​the abdominal window ring 1. 50%;

[0050] The supporting sheet 3 and the cover sheet 2 are made of acrylic material with a thickness of 1.0 mm. The diameters of the cover sheet 2 and the supporting sheet 3 are both smaller than the outer diameter of th...

Embodiment 3

[0052] This embodiment provides a photoacoustic / fluorescence dual-mode imaging method suitable for abdominal organs of animals, the steps include:

[0053] 1) Glue a semicircular acrylic or resin support on the bottom of the ventral window ring to make the ventral window ring into a drawer-like shape;

[0054] 2) Adhere a circular acrylic or resin material with the same diameter as the inner diameter of the abdominal window ring on the surface of the abdominal window, so that it can be perfectly fitted and sealed, and a photoacoustic / fluorescent light suitable for the internal organs of the animal abdominal cavity with the same structure as in Example 1 is obtained. Dual modality imaging device.

[0055] 3) Disinfect and sterilize the device obtained in step 2) by ultraviolet irradiation or wiping with alcohol;

[0056] 4) use the device after step 3) for liver window surgery in C57BL / 6 mice;

[0057] 5) Confocal and photoacoustic microscopy imaging of C57BL / 6 mice treated i...

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Abstract

The invention provides a photoacoustic / fluorescent bimodal imaging method and device suitable for abdominal visceral organs of an animal. The photoacoustic / fluorescent bimodal imaging device is formedby assembling an abdominal window ring and acrylic materials or resin materials (such as CR-39, PC and PMMA) through ingenious design, and the acrylic materials and the resin materials (such as CR-39, PC and PMMA) have excellent light permeability and sound permeability. Meanwhile, the whole photoacoustic / fluorescent bimodal imaging method for the abdominal visceral organs has good biocompatibility and can be simultaneously used for long-term (more than 20 days) dynamic research on the visceral organs through fluorescent confocal and photoacoustic imaging. Used acrylic or resin sheets can stabilize the abdominal visceral organs of the animal in vivo, so that the influence of jittering of the abdominal visceral organs of the animal due to respiration and heartbeat on keeping of the stability of an imaging focal plane is prevented.

Description

technical field [0001] The invention belongs to the technical fields of biological sciences and medical instruments and appliances, and in particular relates to a photoacoustic / fluorescence dual-mode imaging method and device suitable for abdominal internal organs of animals. Background technique [0002] As a newly developed imaging technology, photoacoustic microscopy uses ultrasound as the medium, combining the advantages of high contrast of optical imaging and high penetration of ultrasound imaging. Fluorescence confocal imaging has high imaging contrast and high sensitivity, but it is limited by the serious scattering of light in biological tissues, and it is difficult to break through the hard limit of light transmission. Therefore, it is very necessary to combine the two and learn from each other's strengths for the study of the internal organs of the abdominal cavity of animals. However, the transmission of ultrasound is limited by glass. In actual photoacoustic mic...

Claims

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Application Information

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IPC IPC(8): A61B5/00
CPCA61B5/0071A61B5/0095A61B2503/40
Inventor 张智红邓得强骆清铭
Owner HUAZHONG UNIV OF SCI & TECH