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A kind of Cronobacter dublini for efficiently degrading pyrethroid pesticides and its application

A technology of Cronobacter Dublin and pyrethroids, which is applied in the field of Cronobacter Dublin, can solve the problems of few reports on microecological preparations, the difficulty of the actual degradation ability of strains reaching the laboratory level, and the complexity of the soil environment, etc., to achieve rich Effects of germplasm banks

Active Publication Date: 2022-03-08
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research reports on deltamethrin-degrading strains are limited, and due to the complex soil environment, the actual degradation ability of the strains is difficult to reach the laboratory level, and there are few reports on the preparation of microecological preparations that can be applied to degrade environmental pesticide residues

Method used

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  • A kind of Cronobacter dublini for efficiently degrading pyrethroid pesticides and its application
  • A kind of Cronobacter dublini for efficiently degrading pyrethroid pesticides and its application
  • A kind of Cronobacter dublini for efficiently degrading pyrethroid pesticides and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Isolation, purification and identification of embodiment 1 bacterial strain SM-4

[0020] 1. Screening and isolation of deltamethrin-degrading strains

[0021] Samples were collected from strawberry root soil and sand ginseng root soil polluted by pyrethroid pesticides for a long time in Jintang area, Sichuan Province, and dried naturally through an 80-mesh sieve for later use. Get 1.0g soil sample and add deltamethrin concentration to be the MSM medium of 50mg / L, shake (180rpm) at 35 ℃ after cultivating for 96h, take 5% (v / v) culture fluid to transfer to deltamethrin concentration In the 100mg / L MSM, according to the above operations, the concentration of deltamethrin in the medium was gradually increased to 2000mg / L.

[0022] Above-mentioned basal salt culture medium (MSM) comprises the component of following percentage by weight: (NH 4 ) 2 SO 4 0.05~0.25%, KH 2 PO 4 0.05~0.25%, K 2 HPO 4 0.05~0.25%, MgSO 4 0.05~0.25%, NaCl 0.05~0.25%, distilled water 1000...

Embodiment 2

[0034] Degradation kinetics test of embodiment 2 bacterial strain SM-4 to deltamethrin

[0035] 1. Seed liquid preparation: Pick the Cronobacter dublinii SM-4 colony from the seed slant preserved in Example 1, inoculate it in 50mL LB medium, and cultivate it at an initial pH of 7.0 at 35°C (180rpm) for 24h. Adjust OD with sterile normal saline (0.9% NaCl) 600 To 1.0, it is the seed solution.

[0036] 2. Determination of growth and degradation kinetics: take 5% (v / v) seed solution and inoculate it into 50mL LB medium (containing 75mg / L deltamethrin), use the medium without inoculation as control, and repeat each group of experiments three times. Cultivate at 35°C, 180rpm constant temperature for 5 days, take samples every 2 hours for the first 24 hours, and take samples every 12 hours for the next 84 hours, and use a spectrophotometer to measure the growth of the strain (OD 600 ), adopt Waters2695HPLC chromatographic instrument to analyze and detect its content change of del...

Embodiment 3

[0048] The optimal degradation process condition of embodiment 3 bacterial strain SM-4 deltamethrin

[0049] 1. Test method

[0050] A single factor degradation test was carried out on the strain SM-4, and the degradation rate of deltamethrin by the strain SM-4 was determined by sequentially changing the factors affecting its growth and degradation, such as temperature, pH, degradation concentration, inoculum size, etc. Key factors affecting the degradation efficiency of strain SM-4.

[0051] Utilize Design Expert 12.0 software to carry out experimental design (table 1) according to the principle of response surface method Box-Behnken design, to influence the key factor substrate concentration (X 1 ), temperature (X 2 ), pH(X 3 ) as an independent variable, the degradation rate of deltamethrin with bacterial strain SM-4 as a response value (Y 1 ), to establish a multiple quadratic regression equation. According to the multiple quadratic regression equation for drawing ana...

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Abstract

The invention discloses a Cronobacter dublinensis that efficiently degrades pyrethroid pesticides, named as Cronobacter dublinensis SM-4, and was preserved in the China Committee for the Preservation of Microorganisms on December 17, 2019. General Microbiology Center, the preservation number is CGMCC NO.19218, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The strain can effectively repair the water and soil environment polluted by pyrethroid pesticides, provide a new idea for solving the problem of excessive pyrethroid pesticide residues in agricultural products and environmental pollution, and enrich the germplasm resource bank of pesticide-degrading bacteria. The application prospect is broad.

Description

technical field [0001] The invention relates to the technical field of microbial degradation, in particular to a Cronobacter dublini that efficiently degrades pyrethroid pesticides and an application thereof. Background technique [0002] Pyrethroid pesticides are a class of biomimetic pesticides that are artificially synthesized by extracting the insecticidal compound pyrethrin from natural pyrethrum flowers. It is also one of the successful examples of developing chemical pesticides by simulating the structure of botanical pesticides. In recent years, with the prohibition of most organophosphorus, organochlorine and other pesticides, pyrethroid pesticides have rapidly occupied the market due to their broad-spectrum, high efficiency, and low toxicity, and have become the main pesticides for controlling pests. However, studies have found that pyrethroid pesticides and their metabolites not only remain in water and soil, but are also detected in fruits, vegetables and human u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/38
CPCC02F3/34B09C1/10C02F2101/38C02F2101/306C12R2001/01C12N1/205
Inventor 唐洁雷丹吴敏张庆胡琼
Owner XIHUA UNIV