Preparation method and application of polymer microspheres for protein separation analysis based on core-shell structure

A protein separation and core-shell structure technology, applied in the field of polymer materials, can solve the problems of low separation efficiency, non-uniform particles, poor pressure resistance of chromatographic packing, etc., and achieve the effects of good rigidity, remarkable technical effect and good application prospect.

Active Publication Date: 2020-12-22
苏州博睿嘉晟医疗科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the above-mentioned problems in existing chromatographic packing materials, the present invention provides a method for preparing polymer microspheres with a core-shell structure and its application. The polymer mic

Method used

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  • Preparation method and application of polymer microspheres for protein separation analysis based on core-shell structure
  • Preparation method and application of polymer microspheres for protein separation analysis based on core-shell structure

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Example

[0021]Specific implementation case 1:

[0022]The present invention will be further explained below in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. In addition, it should be understood that after reading the content taught in the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also belong to the protection scope of the claims of this application.

Example Embodiment

[0023]Example 1

[0024]Take 10 ml of newly distilled divinylbenzene, add it to 200 ml of acetonitrile, stir for 30 minutes under nitrogen protection, then add 0.2 g of azobisisobutyronitrile (AIBN), heat to 70°C, and react for 5 hours. Then 3 ml of glycidyl methacrylate (GMA) was added, and the polymerization reaction was continued for 1 hour. The obtained polymer microspheres were thoroughly washed with acetonitrile.

[0025]The above-mentioned microspheres were dispersed in 100 ml of 0.1M (0.1mol / L) dilute sulfuric acid, and the reaction was stirred at 90°C for 2 hours. The obtained microspheres were washed with water and dried. The air-dried microspheres were dispersed in 0.4M (0.1mol / L) NaOH, 10 ml of epichlorohydrin was added, and the reaction was stirred at 50°C for 6 hours. The obtained microspheres were thoroughly washed with ethanol and water.

[0026]The above microspheres were dispersed in a 2% dextran solution, the pH was adjusted to 12.5, and the reaction was carried out at roo...

Example

[0029]Specific implementation case 2:

[0030]The sulfate radical-containing core-shell structure cation exchange polymer microspheres obtained in Example 1 were dispersed in water, and then packed on a 4.6×30 mm chromatographic column for protein separation analysis test. A typical HPLC chromatogram of glycosylated hemoglobin in blood is as followsfigure 2Shown.

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Abstract

The invention relates to a preparation method of polymer microspheres for protein separation analysis based on a core-shell structure, which comprises the following steps: preparing a hydrophobic polymer core with high crosslinking degree and the particle size of 2-10 microns, forming a thin layer of hydrophilic polymer on the surface of the hydrophobic polymer core, and forming a porous hydrophilic polymer gel shell layer with the thickness of 0.1-5 microns. All the layers are connected in a covalent bond mode and the connection is achieved through surface graft copolymerization or surface-initiated active polymerization, surface epoxy ring-opening bonding or substitution reaction of halogen-containing functional groups and the like. The prepared polymer microsphere with the core-shell structure is uniform in particle size, resistant to high pressure and acid and alkali, and the surface can be modified, so that the polymer microsphere containing various surface functional groups is prepared. The synthesized polymer microsphere with the core-shell structure containing various surface functional groups can be applied to chromatographic separation and analysis of biomolecules, especially protein molecules.

Description

technical field [0001] The invention relates to the field of polymer materials, and relates to a polymer microsphere with a core-shell structure, in particular to a method for preparing a polymer microsphere with a core-shell structure with a uniform particle size and suitable for protein chromatographic separation and analysis and the method thereof. application. Background technique [0002] High-performance liquid chromatography (HPLC) is used to separate and analyze biomolecules, especially protein molecules, and plays an increasingly important role in the fields of biomedicine and clinical diagnosis, as well as in life science research. Chromatographic packing is the core and foundation of HPLC technology. In general, chromatography packing materials can be divided into inorganic matrix and organic matrix. Inorganic substrates such as silica gel have the advantages of good rigidity, stable structure, and easy surface modification, and are widely used in HPLC. Organic...

Claims

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Application Information

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IPC IPC(8): C08G81/02C08B37/02C08F212/36C08F220/32B01J20/26B01J20/28B01J20/281B01J20/286B01J20/30G01N30/02G01N30/06
CPCC08G81/02C08B37/0009C08B37/0024C08F212/36B01J20/262B01J20/264B01J20/28021B01J20/286B01J20/281G01N30/02G01N30/06B01J2220/4812B01J2220/52B01J2220/54C08F220/325
Inventor 黄明贤李亚博乔智斌
Owner 苏州博睿嘉晟医疗科技有限公司
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