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A method and system for characterizing protein shape

A protein and characterization technology, applied in the field of analytical chemistry, which can solve the problems of lack of ability to analyze the three-dimensional structure of proteins, laborious and other problems

Active Publication Date: 2021-08-13
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, currently established high-throughput proteomics methods have little capability for protein stereostructure analysis, and structural biology techniques often require laborious steps to obtain high-abundance samples

Method used

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  • A method and system for characterizing protein shape
  • A method and system for characterizing protein shape
  • A method and system for characterizing protein shape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] In this example, the reliability of the method described in the example of the present invention is verified by characterizing the shape of seven globular proteins and one protein dimer.

[0079] Specifically, the diffusion experiments were all carried out on a Lumex CE system (model Capel 105M, St. Petersburg, Russia) equipped with a UV detector (214 nm). The sample is 3kppm insulin, cytochrome C, myoglobin, carbonic anhydrase I, α1 chymotrypsinogen, β-lactoglobulin dimer, ovalbumin, human serum albumin, no need to add neutral markers , the operating system is PH7 disodium hydrogen phosphate-citric acid buffer (5.70mL 0.2mol / L disodium hydrogen phosphate plus 14.30mL 0.1mol / L citric acid buffer), the total length of the capillary is 50cm, and the effective length is 40cm. The inner diameter is 75 μm. The separation pressure is 50mbar, the detection wavelength is 214nm, the injection pressure is 50mbar, the injection time is 5s, and the temperature is 25°C.

[0080] I...

Embodiment 2

[0087] The above-mentioned Example 1 illustrates the reliability of the method provided in the example of the present invention to characterize the protein shape. Since a change in protein conformation can lead to a change in its shape, this method can also be used to distinguish protein conformations. As a proof of concept, protein conformational changes under different pH conditions were investigated using this TDA and MS-based native method. The operating system is 3kppm lysozyme, PH7 and PH3 disodium hydrogen phosphate-citrate buffer (5.70mL 0.2mol / L disodium hydrogen phosphate plus 14.30mL 0.1mol / L citric acid buffer and 16.47mL 0.2 mol / L disodium hydrogen phosphate plus 3.53mL0.1mol / L citrate buffer). Under each voltage condition, three groups of parallel experiments were done. Explore the new method for the characterization of protein conformation changes under different pH conditions. Example 2 and Example 1 use the same experimental conditions, including the same c...

Embodiment 3

[0091] This example studies the conformational changes of BSA at different pH. As an important carrier protein and less likely to aggregate, BSA is usually used as an ideal globular protein for studying the folding and stretching behavior of proteins in chemical denaturation .

[0092] In embodiment 3, the same experimental conditions as in embodiment 2, the same TDA experimental conditions, the same mass spectrometry conditions, and the same simulation conditions are adopted. The sample uses 3kppm of BSA. Each group of experiments was repeated 3 times.

[0093] from Image 6 It can be concluded that the method provided by the embodiment of the present invention can obtain the concentration curve distribution diagram of each component on the basis of separating complex samples, and obtain the diffusion coefficient, particle radius and effective charged amount of each component.

[0094] Experimental results such as Figure 7 . according to Figure 7 It can be seen that B...

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Abstract

The present invention provides a method and system for characterizing the shape of proteins, the method comprising: using an electrospray ion source for sample analysis; obtaining the average charge state Z through the spectrogram av ;according to Z av Determine the solution accessible surface area SASA; according to the SASA and the hydrodynamic volume V of the analyte in the capillary, determine a>c and a

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method and system for characterizing protein shape. Background technique [0002] Native mass spectrometry (native MS) is an extended technique for studying noncovalent protein complexes and protein-protein interactions. On the one hand, natural mass spectrometry has the advantages of very high sensitivity, selectivity, and medium throughput, but provides low-resolution structural information. On the other hand, by diffusion-based correlation techniques, the hydrodynamic radius of proteins can be measured. Both Taylor Diffusion Analysis (TDA) and Dynamic Light Scattering (DLS) can be used for particle radii. However, currently established high-throughput proteomics methods have little capability for protein stereostructure analysis, and structural biology techniques often require laborious steps to obtain high-abundance samples. Contents of the invention [0003] In ord...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N27/622
CPCG01N27/622G01N33/6848G01N2570/00
Inventor 徐伟吴海媚
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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