Method for monitoring polluted water by bioluminescence method

A bioluminescence, water body technology, applied in microorganism-based methods, biochemical equipment and methods, chemiluminescence/bioluminescence, etc., can solve problems such as inability to be practically applied, no proportioning relationship, etc., and achieve the survival rate of bacterial cells. and enzyme activity improvement, broad market application prospects, rapid monitoring of polluted water bodies

Active Publication Date: 2021-01-08
山东省临沂生态环境监测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the resuscitation solution does not have a ratio relationship of specific components, so it cannot be used in practice

Method used

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  • Method for monitoring polluted water by bioluminescence method
  • Method for monitoring polluted water by bioluminescence method
  • Method for monitoring polluted water by bioluminescence method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] The rejuvenation culture solution of Photobacterium ichthyosa for monitoring environmental pollutants has the following components:

[0070] Sodium chloride 26g / L,

[0071] Magnesium chloride 6g / L,

[0072] Adenosine triphosphate 4g / L,

[0073] Methionine 1g / L,

[0074] Calcium acetate 0.8g / L,

[0075] Sterile defibrinated sheep blood 35ml / L.

[0076] Preparation method: take each raw material, add to distilled water in turn, mix well, and sterilize to obtain.

Embodiment 2

[0078] The rejuvenation culture solution of Photobacterium ichthyosa for monitoring environmental pollutants has the following components:

[0079] Sodium chloride 18g / L,

[0080] Magnesium chloride 5g / L,

[0081] Adenosine triphosphate 3g / L,

[0082] Methionine 1g / L,

[0083] Calcium acetate 0.5g / L,

[0084] Sterile defibrinated sheep blood 30ml / L.

Embodiment 3

[0086] The rejuvenation culture solution of Photobacterium ichthyosa for monitoring environmental pollutants has the following components:

[0087] Sodium chloride 20g / L,

[0088] Magnesium chloride 6g / L,

[0089] Adenosine triphosphate 5g / L,

[0090] Methionine 2g / L,

[0091] Calcium acetate 1g / L,

[0092] Sterile defibrinated sheep blood 50ml / L.

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Abstract

The invention belongs to the technical field of biological environment monitoring, and discloses a method for monitoring polluted water by a bioluminescence method. The method comprises the followingsteps: (1), preparing a rejuvenation culture solution; (2), rejuvenating photogenic bacteria; and (3), monitoring sewage. The method is rapid and sensitive in polluted water monitoring and wide in market application prospect.

Description

technical field [0001] The invention belongs to the technical field of biological environment monitoring, and in particular relates to a bioluminescent method for monitoring polluted water bodies. Background technique [0002] In recent years, with the improvement of the industrialization process and the great demand for chemical products, the number of various chemical substances has increased sharply, which has greatly affected the living environment of human beings. It is urgent to identify the acute toxicity of the increasing environmental pollutants. Environmental monitoring methods can be divided into two categories: physical and chemical analysis techniques and biological monitoring. Among them, physical and chemical analysis techniques are often used to test a single physical and chemical index, but they cannot reflect the toxicity of pollutants. Traditional biological monitoring uses daphnia, algae or fish as the test objects. Although it can reflect the direct imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N21/64C12N1/20C12R1/01
CPCG01N21/763G01N21/6428G01N21/6486C12N1/20
Inventor 马云朱文霞
Owner 山东省临沂生态环境监测中心
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