Lactobacillus plantarum kbl396 strain and use thereof
A technology of Lactobacillus plantarum and strains, applied in the directions of Lactobacillus plantarum, bacteria, application, etc., can solve problems such as drug treatment
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Embodiment 1
[0094] Example 1. Screening of probiotic strains capable of inducing serotonin biosynthesis
[0095] In many previous literatures, serotonin has been reported to play an important role in neurological diseases, especially mental disorders. Using 47 probiotic strains (19 Lactobacillus spp., 28 Bifidobacterium spp.) isolated from infant and adult feces and 2 strains sold as probiotic products Lactobacillus plantarum, the ability to induce serotonin biosynthesis was assessed. Induction of serotonin biosynthesis was confirmed by treating each strain to the enterochromaffin cell line RIN14B and observing how much the expression of the rate-limiting enzyme TPH-1 (tryptophan hydroxylase-1) gene of the serotonin biosynthetic pathway was elevated Ability.
[0096] 1-1. Culture of strain and preparation of culture supernatant
[0097] In order to screen for novel strains shown to be effective against neurological diseases, 47 candidate lactic acid bacteria isolated from the feces of ...
Embodiment 2
[0115] Example 2. Preparation of Feed Comprising KBL396 Strain and Feed Administration in Animal Models
[0116] It was confirmed in Example 1 that the KBL396 strain has an excellent effect on serotonin biosynthesis in vitro. In order to confirm whether the KBL396 strain has an excellent effect on nervous system diseases in vivo, experiments were performed using animal models through preclinical experiments. To this end, a feed containing the KBL396 strain was prepared and administered in an animal model.
[0117] 2-1. Feed preparation
[0118] Since oral administration causes a great deal of stress to experimental animals and this stress may affect the objectivity (bias) of the experimental results, experiments were planned for animal models administered in the form of feed prepared by mixing strains.
[0119] Feed for strain administration was prepared by the following method. The KBL396 strain was activated by culturing in MRS medium for 24 hours, and subcultured by inocu...
Embodiment 3
[0123] Example 3. Effect of improving cognitive ability by administering KBL396 strain (Y-maze (MAZE) experiment)
[0124] 28 days after the administration of the strain, the effect of improving cognitive ability caused by KBL396 treatment was tested by performing a Y-maze test. The animal model used in the present invention was 7-week-old C57BL / 6 mice, and experiments were performed using 8 mice in each of the PBS control group and the KBL396-treated group.
[0125] The Y-maze test is a behavioral experiment designed to judge cognitive abilities using animal models. In the experiment, the three arms of the Y maze are connected to each other at an angle of 120°, and each arm is named A, B, and C, respectively. The experimental animals are placed in one arm and allowed to move freely for 8 minutes, and then mounted on the ceiling using A camera and a computer program connected to it observe and record the number and sequence of entries into each arm. Then, when the tail was f...
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