Unlock instant, AI-driven research and patent intelligence for your innovation.

Compound euphorin F and application in preparation of anti-tomato yellow leaf curl virus drug

A technology of tomato yellow leaf curl and compounds, which is applied in the field of preparation of anti-tomato yellow leaf curl virus drugs, can solve the problems of plant poisoning, tomato production area reduction, and unclear anti-virus mechanism, etc.

Active Publication Date: 2021-01-26
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Resistance to tomato yellow leaf curl virus is one of the main diseases in tomato production. Every year, resistance to tomato yellow leaf curl virus (TYLCV) will cause a large number of tomato production areas to reduce or even eliminate harvests, causing huge losses to farmers
Most of the anti-TYLCV preparations currently on the market can only prevent the infection of the virus and delay or reduce the development of symptoms. Once the plant is infected, it is difficult to fundamentally reduce the losses caused to production, and the ingredients are generally It is mixed with crude extracts extracted from plants, and the specific antiviral mechanism is not clear
Although commercially available chemical synthetic agents have clear chemical components and have a relatively good effect of inhibiting virus replication, they will also cause toxicity to the plants themselves when sprayed at a slightly higher concentration, so they still cannot solve the problem of tomato yellow leaf curl virus. production problems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound euphorin F and application in preparation of anti-tomato yellow leaf curl virus drug
  • Compound euphorin F and application in preparation of anti-tomato yellow leaf curl virus drug
  • Compound euphorin F and application in preparation of anti-tomato yellow leaf curl virus drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Extraction and separation of compound 6:

[0023] Separation methods such as figure 2 shown.

[0024] The traditional Chinese medicine Euphorbia euphorbia (10 kg) purchased from the medicinal material market in Urumqi, Xinjiang was refluxed three times with methanol (4, 3 and 3 hours respectively). extraction. The ethyl acetate part (2.3kg) was chromatographed on a silica gel column, eluted with petroleum ether: ethyl acetate gradient (from 1:0 to 0:1), and was divided into seven parts (Fr.1-7), No. 5 parts (175g) were separated into 12 parts by C18 reverse-phase silica gel column chromatography (CH3OH-H2O from 3:7 to 9:1), and the second part of this section, Fr.5B (8g), was subjected to methanol gel ( Sephadex LH-20) separation. Two fractions (Fr.5B1-Fr.5B2) were obtained. Compound 6 (11.3 mg) was obtained from Fr. 5B2 (0.8 g) by semi-preparative HPLC. Structural formula such as figure 1 shown.

[0025] Euphorin F(6): white amorphous powder; [a] 25 D+6.1(c 0...

Embodiment 2

[0028] Anti-TYLCV activity assay method:

[0029] Compound 6 was made into a solution, and 4-6 leaf-age N. benthamiana plants were used as the experimental object. The drug solution was sprayed on the tobacco leaves for 6 hours, and then the TYLCV virus was inserted. And after 10 days, the virus load in the systemically infected leaves was evaluated by the CTAB (acetylmethyl bromide) method. Viral DNA in infected N. benthamiana leaves was detected by real-time quantitative PCR. Compounds and positive controls were replicated in triplicate. The activity of compound 6 against tomato yellow leaf curl virus (TYLCV) was evaluated, and it was found that compound 6 had significant activity against tomato yellow leaf curl virus at a concentration of 40 mol / mL, and the inhibition rate was 72.0%. The inhibition rate of positive control Ningnanmycin was 57.1%. The comparison of compound 6 of the present invention and Ningnanmycin on the infection inhibition rate of TYLCV on Nicotiana ...

Embodiment 3

[0031] Preparation of Compound Formulations

[0032] Compound 6 was dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg / mL, and then diluted with sterile water to 40 μg / mL for activity screening. Ningnanmycin was diluted with sterile water to a concentration of 80 μg / mL according to the instructions.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a compound euphorin F and application in preparation of anti-tomato yellow leaf curl virus drugs. The invention relates to a compound euphorin F, a pesticide composition of thecompound euphorin F and application of the compound euphorin F in preparation of anti-tomato yellow leaf curl virus drugs. According to the invention, the tomato yellow leaf curl virus (TYLCV) resisting activity evaluation results of the compound euphorin F show that when the concentration of the compound euphorin F is 40 mol / mL, the compound euphorin F has the remarkable tomato yellow leaf curl virus resisting activity, and the inhibition rate is 72.0%, wherein the inhibition rate of the positive control ningnanmycin is 57.1%.

Description

technical field [0001] The invention belongs to the technical field of pesticides, and in particular relates to compound euphorin F (compound 6) and its application in the preparation of anti-tomato yellow leaf curl virus medicine. Background technique [0002] Plant virus is a kind of plant disease second only to fungi and bacteria. It is very harmful and difficult to control. It is called "plant cancer". Resistance to tomato yellow leaf curl virus (TYLCV) is one of the main diseases in tomato production. Every year, resistance to tomato yellow leaf curl virus (TYLCV) will cause a large number of tomato production areas to reduce or even eliminate harvests, causing huge losses to farmers. Most of the anti-TYLCV preparations currently on the market can only prevent the infection of the virus and delay or reduce the development of symptoms. Once the plant is infected, it is difficult to fundamentally reduce the losses caused to production, and the ingredients are generally I...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07J1/00A01N45/00A01P1/00
CPCA01N45/00C07J1/0011
Inventor 李顺林赵宁东龙琴杨宝嘉
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI