Composite probiotic preparation for improving low pH condition of rumens of dairy cows and application of composite probiotic preparation to dairy cow breeding
A technology of compound probiotics and probiotics, applied in the application, bacteria, microorganisms and other directions, can solve the problems affecting the breeding efficiency of dairy cows, reduce the digestibility of feed, metabolic diseases, etc. The effect of increased nutrients
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Embodiment 1
[0033] The composite effect of 10 yeast strains was studied by in vitro fermentation. Feed with a fine-to-concentrate ratio of 65:35 was used as the ration of rumen fluid donor cattle and the substrate for fermentation, and the total concentration of yeast added was 1×10 8 CFU·30mL -1(various yeasts and other viable counts mixed) under the conditions of rumen syringe fermentation test in vitro (refer to Menke, K.H., Raab, L., Salewski, A., Steingass, H., Fritz, D., Schneider, W. The estimation of the digestibility and metabolizable energy content of ruminant feedingstuffs from the gas production when they are incubated with rumen liquor in vitro. The Journal of Agricultural Science, 1979,93(1):217-222.), by studying the effect of different compound strains on fermentation broth pH (directly measured by pH meter), feed degradation rate (calculated based on the percentage of feed degradation), NH 3 -N concentration (determined by sodium nitroferricyanide-sodium hypochlorite co...
Embodiment 2
[0052] On the basis of the excellent yeast composite bacterial strain obtained by screening in Example 1, carry out in vitro fermentation experiments with 5 strains of bacteria respectively, the experimental method is the same as Example 1, and the viable bacterial concentration of bacteria is 1 × 10 8 CFU·30mL -1 , the total viable yeast concentration was 1×10 8 CFU·30mL -1 , the total concentration of probiotics in the in vitro fermentation experiment system was 2×10 8 CFU·30mL -1 .
[0053] Yeast combinations used in this test: Y3+Y8(YT), Y3+Y4+Y5+Y6+Y7+Y8+Y9(YS)
[0054] The names and numbers of the bacterial strains used in this test are shown in Table 4.
[0055] Table 4 Bacterial strain names and numbers used
[0056]
[0057]
[0058] Effects of Yeast and Bacteria Combination on Rumin Fermentation in Vitro
[0059] It can be seen from Table 5 that the compound yeasts YT and YS respectively combined with bacteria B1, B2, B3, B4 and B5 had different effects ...
Embodiment 3
[0074] The experimental animals were 40 Chinese Holstein dairy cows, selected from 153 lactating cows raised by Xinzhou Yinshan Lake Dairy Farming Co., Ltd. The experiment adopted a block design, and the dairy cows with the same body condition and parity, lactation days and milk production were classified into one block, and 40 Holstein cows were selected and divided into 8 blocks. Group 5 Holstein dairy cows, and randomly divide 5 Holstein dairy cows in each block group into I, II, III, IV and CK groups, so that each group has 8 experimental dairy cows, and the test period is 70 days. The period is 10d, and the feeding period is 60d. The milk production was recorded every day, and the milk samples of each group were collected before the experiment, and the feed intake and digestibility were measured. The specific groups are as follows:
[0075] Test group I: (Y3+Y8)+B3+basal diet
[0076] Test group II: (Y3+Y4+Y5+Y6+Y7+Y8+Y9)+B2+basal diet
[0077] Test group III: (Y3+Y8)...
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