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Sample pad for separating and concentrating target and uses thereof

A technology of sample pads and target objects, which is applied in the field of biochemical testing, can solve the problems that the potential of pretreatment tools has not been brought into play, and achieve the effects of reducing the amount of reagents, improving detection efficiency, and reducing applications

Pending Publication Date: 2021-02-19
SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the potential of the sample pad as a sample preparation tool is not realized

Method used

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  • Sample pad for separating and concentrating target and uses thereof
  • Sample pad for separating and concentrating target and uses thereof
  • Sample pad for separating and concentrating target and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1. prepares modified sample pad by immersion physisorption method

[0049] Use tweezers to soak the non-woven fabric sample pad in a methanol solution containing 10 mM 2,2'-(octadecylimino)diethanol for 30 minutes, then place the sample pad in a porous grid rack and dry it at 70°C for 8 Hour. Afterwards, the sample pad is cut into a rectangular block of 4.8×18 mm to obtain a sample pad with long fatty chains on the surface.

Embodiment 2

[0050] Example 2. Preparation of modified sample pads by soaking in situ generation method

[0051] Cut the glass fiber sample pad into small pieces of 4.8×18mm, put them into a Teflon mesh bag, and suspend them in 200mL of a mixture of polystyrene, acrylic acid and potassium persulfate (5g of polystyrene, 5g of acrylic acid and Potassium persulfate (0.625g) in deionized water, after nitrogen exchange, was heated up to 75°C and reacted overnight. After the reaction, the mesh bag was taken out, and the glass fiber was dried at 90° C. for 24 hours to obtain a polystyrene-modified sample pad with carboxyl groups on the surface.

[0052] Cut the non-woven sample pad into small pieces of 4.8×18mm, fix it on the wall of a 500mL beaker with a magnet, add 200mL of 10mM ethanol solution of 2-phenylethyltrichlorosilane into the beaker, heat and stir under magnetic force Stir in a container and heat to 60°C. Then, 7.5 mL of 0.1M ammonia water was added dropwise thereto, keeping 1 drop / ...

Embodiment 3

[0053] Example 3. Preparation of silanol-modified sample pads by coating physical adsorption

[0054] Grind 5g of 300-mesh to 400-mesh silicon dioxide, 30ml of water and 0.5% sodium carboxymethylcellulose in a mortar for 30 minutes, pour it on the glass fiber sample pad, spread it flat with a spreader, and let it air in the air After drying for 48 hours and drying at 60° C. for 2 hours, a sample pad whose surface is modified with silanol can be obtained.

[0055] The preparation method of the non-woven fabric sample pad modified by silanol is similar, the only difference is that the non-woven fabric is used as the sample pad.

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PUM

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Abstract

The invention belongs to the field of biochemical examination. More specifically, the present invention provides a sample pad for separating and concentrating a target, the sample pad being modified by chemical groups so as to be capable of separating and concentrating the target by adsorption. The sample is pretreated by the sample pad, the pretreatment process of the sample is simplified, and consumables for sample pretreatment and the consumption of a target object are reduced, so that the detection efficiency is effectively improved, and the method is more economical and environment-friendly. The invention also provides a method for separating and concentrating a target object by using the sample pad, and an immunochromatographic test strip comprising the sample pad, The invention alsodiscloses application of the sample pad in immunochromatographic analysis and mass spectrometric detection analysis.

Description

technical field [0001] The invention belongs to the field of biochemical testing; more specifically, the present invention relates to a sample pad for separating and concentrating a target, an immunochromatographic test strip comprising the sample pad, and the sample pad in immunochromatographic analysis Or the application of mass spectrometry detection analysis. Background technique [0002] As we all know, in the detection, the concentration of the target substance in the sample liquid determines the detection sensitivity. As in immunochromatography, the concentration of the target that contacts the antibody on the nitrocellulose (NC) membrane determines the detection sensitivity. [0003] The use of existing immunochromatographic test strips generally involves pretreatment of the sample, converting the target into a test solution by various means, and then using the sample pad at the front end of the immunochromatographic test strip to absorb the liquid and divert it to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/34G01N1/40G01N33/558
CPCG01N1/28G01N1/34G01N1/405G01N33/558
Inventor 严义勇马红圳邓炀
Owner SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD